Xiaorong Gu, Ph.D.

Research Associate

Department of Ophthalmic Research, Cole Eye Institute
Telephone: (216) 445-0424
Fax : (216) 445-3670

ACADEMIC AND PROFESSIONAL TRAINING:

B.S. 1993, Sichuan University, Sichuan, P.R. China - Chemical Engineering
M.S. 1996, Sichuan University, Sichuan, P.R. China - Organic Chemistry
Ph.D.  2002, Case Western Reserve University, Cleveland, OH. Department of Chemistry

PROFESSIONAL EXPERIENCE:

1992 – 1993 Research Assistant, Sichuan University, Chengdu, Sichuan, P.R.China.
1993 – 1994 Teaching Assistant, Sichuan University, Chengdu, Sichuan, P.R.China.
1993 – 1996 Research Assistant, Sichuan University, Chengdu, Sichuan, P.R.China.
1997 – 1998 Teaching Assistant, Dept Cehmistry, Case Western Reserve University, Cleveland, OH. USA
1998 – 2002 Research Assistant, Dept Chemistry, Case Western Reserve University, Cleveland, OH. USA
1999 – 2002 Research Assistant, Cole Eye Institute, Cleveland Clinic Foundation, OH. USA.
2002-2005 Postdoctoral Fellow, Department of Ophthalmic Research. The Cleveland Clinic Foundation, Cleveland, OH
2006-Present Research Associate, Department of Ophthalmic Research. The Cleveland Clinic Foundation, Cleveland, OH

PUBLICATIONS:

Guo SJ, Luo MM, Gu X, Xie RG, Zhao HM, (1995), The design and synthesis of imidazolium cyclophanes. Chin Chem Lett. 6:203.

Zhou CH, Gu X, Xie RG, et al. (1999), Convenient and efficient synthesis for a series of ether bis-imidazoles and their derivatives. Synthetic Commun 29:7 1217-1222

Crabb JW, M Miyagi, X Gu, K Shadrach, KA West, H Sakaguchi, M Kamei, A Hasan L Yan, ME Rayborn, RG Salomon, JG Hollyfield (2002) Drusen Proteome Analysis: an Approach to the Etiology of Age-Related Macular Degeneration. Proc Natl Acad Sci USA 99:14682-14687.

Gu X, Sun M, Gugiu B, Hazen S, Crabb JW, Salomon RG. (2003) Oxidatively Truncated Docosahexaenoate Phospholipids: Total Synthesis, Generation, and Peptide Adduction Chemistry. J Org Chem. 68(10): 3749-61.

Gu X, Meer SG, Miyagi M, Rayborn ME, Hollyfield JG, Crabb JW, Salomon RG. (2003). Carboxyethylpyrrole protein adducts and autoantibodies, biomarkers for age-related macular degeneration. J Biol Chem. 278(43):42027-35.

Bhattacharya SK, JS Crabb, SP Annangudi, KA West, X Gu, J Sun, VL Bonilha, G Smejkal, K Shadrach, JG Hollyfield and JW Crabb (2006) “Optic Nerve Fractionation for Proteomics” In: SEPARATION METHODS IN PROTEOMICS (GB Smejkal and A Lazarev, eds), CRC Taylor & Francis, Boca Raton, FL (pp 135-156).

Bhattacharya SK , KA West, X Gu, JS Crabb, K Renganathan, Z Wu, J Sun and JW Crabb (2006) “Fractionation of Retina for Proteomic Analyses” In: SEPARATION METHODS IN PROTEOMICS (GB Smejkal and A Lazarev, eds), CRC Taylor & Francis, Boca Raton , FL (pp 157-186).

Bonilha VL, ME Rayborn , SK Bhattacharya , X Gu, JS Crabb, JW Crabb, and JG Hollyfield (2006) “The Retinal Pigment Epithelium Apical Microvilli And Retinal Function” In : RETINAL DEGENERATIVE DISEASES (JG Hollyfield, and RE Anderson, MM LaVail, eds), Springer, New York (pp519-524).

Gugiu, B.G.; Mesaros, A. C.; Sun, M.; Gu, X .; Crabb, J.W.; Salomon, R.G. (2006) Identification of oxidatively truncated ethanolamine phospholipids in retina and their generation from polyunsaturated phosphatidylethanolamines. Chem Res Toxicol. 19: 262-71

Bhattacharya SK , Crabb JS, Bonilha VL, Gu X, Takahara H, and Crabb JW (2006) Proteomics Implicates Peptidyl Arginine Deiminase 2 and Optic Nerve Citrullination in Glaucoma Pathogenesis. Invest Ophthalmol Vis Sci. 47(6):2508-14.

Organisciak D, R Darrow, X Gu, L Barsalou, and JW Crabb (2006) Genetic, Age and Light Mediated Effects on Crystallin Protein Expression in the Retina. Photochem and Photobiol 82: 1088-1096.

Ebrahem Q, K Renganathan, J Sears, A Vasanji, X Gu, L Lu, RG Salomon, JW Crabb, B Anand-Apte (2006) Carboxyethylpyrrole Oxidative Protein Modifications Stimulate Neovascularization: Implications for Age-Related Macular Degeneration. Proc Natl Acad Sci USA 103: 13480-13484.

ABSTRACTS:

Crabb JW, Yan L, West KA, Crabb JS, Marmorstein A, Gu X, Kamei M, Rayborn ME, Hollyfield JG, Salomon RG. (2000), Proteomic Approaches to the Study of Retinal Degenerative Disease. Invest. Opththal. Vis.Sci. 41:4, 3250.

Gu X, Miyagi M, Rayborn RE, Hollyfield JG, Salomon RG, Crabb JW. (2001) Detection and Characterization of Protein Adducts from oxidation of Docosahexaenoate phospholipids Using Antibodies and Mass Spectrometry. Annual Residents and Alumni Meeting of Cole Eye institute, Cleveland Clinic Foundation.

Gu X, Miyagi M, Sun M, Gugiu B, Crabb J.W, Salomon RG. (2001) Oxidation of Docosahexaenoate Phospholipids: An Electrospray Mass Spectroscopic Study. 222nd American Chemical Society National Meeting, Chicago, USA.

Gu X, Miyagi M, Sun M, Gugiu B, Rayborn RE, Hollyfield JG, Salomon RG, Crabb JW. (2001) Protein Modification from Oxidation of Docosahexaenoate phospholipids. Proceedings of the 49th American Society of Mass Spectrometry Conference on Mass Spectrometry and Allied Topics.

Gu X, Shadrach K, Sun M, West KA, Hazan SL, Salomon RG, Hollyfield JG and Crabb JW. (2003) Characterization of Lipid Oxidation Products and Proteins in Bruch’s Membrane from Normal and AMD Donor Eyes. ABRF 2003, Translating biology Using Proteomics and Functional Genomics, February 10-13, 2003, Denver, CO, J Biomolecular Techniques 14: 88, P137-T.

Gu X, Sun M, Gugiu B, West KA, Shadrach K, Organisciak D, Hazen S, Hollyfield JG, Salomon RG, Crabb JW. (2003) Characterization of Oxidatively Modified Lipid and Protein Adducts in Bruch's Membrane Using Mass Spectrometry and Antibodies. 2003 ABRF conference. 

Gu X, K Shadrach, M Sun, KA West, L Shan, S Hazan, RG Salomon, JG Hollyfield and JW Crabb. (2003) Identification of Lipid Oxidation Products and Proteins in Bruch’s Membrane from Normal and AMD Donor Eyes. Annual Meeting of the Association for Research in Vision and Ophthalmology, May 4-9, 2003, Ft. Lauderdale, FL. Abstract 1622

Renganathan K, Sun M, Darrow R, Shan L, Gu X, Salomon RG, Hazen S, Organisciak D and Crabb JW. (2003) Light Induced Protein Modifications and Lipid Oxidation Products in Rat Retina. Annual Meeting of the Association for Research in Vision and Ophthalmology, May 4-9, 2003, Ft. Lauderdale, FL, Abstract 5129.

Crabb JW, Gu X, Renganathan K, Miyagi M, Ebrahem Q, Anand-Apte B, Hazen SL, Organisciak DT , Salomon RG and Hollyfield JG. (2003) Oxidative Protein Modifications and Age-Related Vision Loss. Sixth International Synposium on Mass Spectrometry in the Health & Life Sciences, San Francisco CA, August 24-28, 2003.

West KA, Gu X, Renganathan K, Sun M, Darrow R, Hazen S, Salomon RG, Organisciak D and Crabb JW. (2003) Light Induced Protein Modifications and Lipid Oxidation Products in Rat Retina. Great Lakes Vision Research Conference, Miami University, Oxford OH, October 17-18, 2003.

Sun J, Gu X, Shadrach K, West KA, Sun M, Hazan S, Salomon RG, Hollyfield JG and Crabb JW. (2003) Identification of Lipid Oxidation Products and Proteins in Bruch’s Membrane from Normal and AMD Donor Eyes. Great Lakes Vision Research Conference, Miami University, Oxford OH, October 17-18, 2003.

Renganathan K, Ebrahem Q, Gu X, Salomon RG, Anand-Apte B and Crabb JW. (2003) Carboxyethylpyrrole Protein Modifications Stimulate Angiogenesis. Great Lakes Vision Research Conference, Miami University, Oxford OH, October 17-18, 2003.

Renganathan K, R Collier, X Gu, RG Salomon, M Kapin, JG Hollyfield and JW Crabb (2004) Similarities in Oxidative Damage from AMD and Retinal Light Damage. Annual Meeting of the Association for Research in Vision and Ophthalmology, April 25-29, 2004, Ft. Lauderdale, FL. Abstract 1795.

Gu X, K Renganathan, C Grimm, A Wenzel, RG Salomon, CE Reme, and JW Crabb (2004) Rapid Changes in Retinal Oxidative Protein Modifications Induced by Blue Light. Annual Meeting of the Association for Research in Vision and Ophthalmology, April 25-29, 2004, Ft. Lauderdale, FL. Abstract 3474.

Anand-Apte B, K Renganathan, Q Ebrahem, X Gu, RG Salomon and JW Crabb (2004) Carboxyethylpyrrole Protein Modifications Stimulate Angiogenesis. Annual Meeting of the Association for Research in Vision and Ophthalmology, April 25-29, 2004, Ft. Lauderdale, FL. Abstract 483.

Gu J, X Gu, RG Salomon, H Lewis, SA Hagstrom, and JW Crabb (2004) Biomarkers for Age-Related Macular Degeneration. Annual Meeting of the Association for Research in Vision and Ophthalmology, April 25-29, 2004, Ft. Lauderdale, FL. Abstract 1831.

Crabb JW, SK Bhattacharya, JS Crabb, KA West, J.Sun, JC Saari, VL Bonilha (2004) Support For a Retinoid-Processing Complex In Apical RPE. Annual Meeting of the Association for Research in Vision and Ophthalmology, April 25-29, 2004, Ft Lauderdale, FL. Abstract 4589.

Crabb JW, X Gu, K West, N Renganthan, J Gu, R Salomon, B Anand-Apte, DT Organisciak, and JG Hollyfield (2004) Molecular Mechanisms Underlying Age-Related Macular Degeneration. The 15 th Meeting of Methods in Protein Structure Analysis, August 29-September 2, 2004, Seattle, WA.

Gu X, Crabb JS, Annangudi SP, Bonilha VL, Shadrach K, Hollyfield JG, Bhattacharya SK, Crabb JW (2005) Optical Nerve Fractionation for Proteomic Analysis. Annual Meeting of the Association for Research in Vision and Ophthalmology, May 1-5, 2005, Ft Lauderdale, FL. Abstract 1255.

Gugiu BG, Rozanowska M, Rozanowski B, Rayborn ME, Bonilha VL, Gu X, Salomon RG, Hollyfield JG, Boulton ME, Crabb JW (2005) Proteomic and Ultrastructural Analyses of Human Lipofuscin. Annual Meeting of the Association for Research in Vision and Ophthalmology, May 1-5, 2005, Ft Lauderdale, FL. Abstract 1739.

Bonilha VL, Rayborn ME, Gu X, Bhattacharya SK, Crabb JS, Crabb JW, Hollyfield JG (2005) Characterization of M6a in the Retinal Pigment Epithelium Microvilli. Annual Meeting of the Association for Research in Vision and Ophthalmology, May 1-5, 2005, Ft Lauderdale, FL. Abstract 3051.

Gu J, Wu Z, Crabb JS, Gu X, Hagstrom SA, Sears JE, Lewis H, Salomon RG, Crabb JW, Cleveland AMD Study Group. (2005) Plasma Peptidomics for Predicting AMD Susceptibility. Annual Meeting of the Association for Research in Vision and Ophthalmology, May 1-5, 2005, Ft Lauderdale, FL. Abstract 2998.

Renganathan K, Gu J, Gugiu B, Gu X, Darrow R, Lewis H, Salomon RG, Organisciak DT, Crabb JW, Cleveland AMD Study Group (2005) Similarities in Plasma Oxidative Modifications From Retinal Light Damage and AMD. Annual Meeting of the Association for Research in Vision and Ophthalmology, May 1-5, 2005, Ft Lauderdale, FL. Abstract 3010.

Bhattacharya SK, Crabb JS, Gu X, Crabb JW (2005) Proteomics Suggests Glaucoma Pathogenesis May Involve Optic Nerve Peptidylarginine Deiminase. (2005) Annual Meeting of the Association for Research in Vision and Ophthalmology, May 1-5, 2005, Ft Lauderdale, FL. Abstract 3510.

Crabb JS, Renganathan K, Gu X, West KA, Bhattacharya SK, Wu Z, Crabb JW (2005) Fractionation of Retina for Proteomic Analysis. Annual Meeting of the Association for Research in Vision and Ophthalmology, May 1-5, 2005, Ft Lauderdale, FL. Abstract 5154.

Crabb JW, J Gu 1 , Z Wu, JS Crabb, X Gu, E Simpson, E Bala, G Sturgill, S Yaniglos, N Peachey , SA Hagstrom, P Kaiser, J Sears, H Lewis, and RG Salomon. (2005) Using Proteomics to Predict AMD Susceptibility. 2nd SERI-ARVO Meeting on Research in Vision and Ophthalmology. 16-20 February 2005, Singapore . Am J Ophthalmol 139: S1-74.

Crabb JS, Gu X, Nawrot M, Saari JC, Crabb JW (2006) Quantitative Mass Spectrometric Analysis of CRALBP-Protein Interactions. Annual Meeting of the Association for Research in Vision and Ophthalmology, April 30-May 4, 2006, Ft Lauderdale, FL. Abstract 2039.

Gu J, Crabb JS, Bala E, Renganathan K, Gu X, Hagstrom SA, Lewis H, Salomon RG, Crabb JW, Cleveland AMD Study Group (2006) Peptidomic Profiling of Plasma for AMD Biomarkers. Annual Meeting of the Association for Research in Vision and Ophthalmology, April 30-May 4, 2006, Ft Lauderdale, FL. Abstract 4173.

Gu X, Crabb JS, Nawrot M, Saari JC, Crabb JW (2006) Quantitative Mass Spectrometric Analysis of Visual Cycle Protein Interactions. Annual Meeting of the American Society for Mass Spectrometry, May 28-June 1, 2006, Seattle, WA. Poster 616.