Proteins in Proliferating ARPE19 RPE Cells (Gel 26C)

Spot # Protein NCBInr # Peptide Sequence On Gel Theoretical
    Accession # Matches Coverage % MW (kDa) pI MW (kDa) pI
                 
1 alpha-Crystallin B chain 4503057 8/32 40% 25 6.7 20.16 6.8
2 Triosephosphate isomerase 136060 7/26 40% 28 6.6 26.67 6.5
3 Phosphoglycerate mutase 2 4505753 6/22 34% 30 6.6 28.80 6.7
4 Phosphoglycerate mutase 2 4505753 7/27 46% 30 6.2 28.80 6.7
5 Antioxidant protein 2 4758638 5/29 25% 25 6.0 24.90 6.0
6 Heat Shock 27kD Protein (HSP 27)  4504517 8/42 42% 30 5.8 22.78 6.0
7 Glutathione S-transferase 2204207 9/28 55% 25 5.3 23.38 5.4
8 Glyceraldehyde-3-phosphate dehydrogenase (GPDH) 136060 7/22 44% 40 8.5 36.09 8.7
9 Phosphoglycerate kinase 1 4505763 8/20 28% 45 8.5 45.00 8.6
10 Cellular retinaldehyde-binding protein (CRALBP) 4506541 10/26 34% 35 8.0 36.47 5.0
11 Heterogeneous nuclear ribonucleoprotein A2/B1 4504447 5/18 18% 35 7.7 37.43 8.7
12 Annexin II (Lipocortin II) 4757756 12/26 32% 38 7.0 38.60 7.6
13 alpha-Enolase 4503571 10/29 33% 50 7.0 47.38 7.0
14 Annexin V (Lipocortin V) 4502170 10/20 37% 35 4.7 35.81 4.9
15 gamma-Enolase  2118304 10/31 39% 50 4.5 44.20 4.9
16 Cytokeratin 18 4557888 8/21 30% 47 5.2 47.33 5.3
17 Cytokeratin 8 4504919 10/22 27% 55 5.4 53.56 5.5
18 Protein disulfide isomerase (ER-60 protease) 2507461 10/33 25% 60 5.5 57.18 6.1
19 ATP Synthase beta chain 114549 16/27 50% 55 5.0 56.56 5.3
20 Prolyl 4-hydroxylase beta subunit 2507460 12/26 34% 58 4.8 57.12 4.8
21 Heat Shock 60 kD Protein 1(Mitochondrial matrix protein P1 precursor) (60kD Chaperoin, HSP60) 129379 10/23 28% 60 5.2 61.23 5.7
22 Actin 481515 14/33 45% 42 5.0 42.00 5.3
23 78 kD Glucose-regulated protein (GRP-78)(BIP) 6470150 10/26 23% 70 4.8 72.23 4.9
24 Heat shock 70kD protein 10 (Heat shock cognate 71 kD protein) 5729877 8/22 22% 70 5.3 70.90 5.4
25 Tumor rejection antigen 1 ( Endoplasmin Precursor) (94kD Glucose-Reculated Protein) (GRP94) 121567 9/28 16% 90 4.5 92.47 4.8
26 alpha-Actinin 4 2804273 15/36 22% 100 5.2 102.27 5.3
27 Actin dimer 481515 8/29 31% 100 5.3 42.14 5.3
28 Vimentin Dimer 2119204 13/46 30% 100 5.0 53.65 5.1
29 Vimentin Dimer 2119204 14/26 41% 100 5.0 53.65 5.1
30 Single-minded homolog 1 (hSIM1) 3024618 8/29 16% 80 6.0 85.47 7.0
31 Vinculin 4507877 6/29 12% 120 5.7 116.72 5.8
32 beta-Tubulin 2119276 12/29 29% 50 5.0 49.76 4.8
33 Nucleophosmin (Nucleolar phosphoprotein B23)  114762 6/23 24% 40 4.5 32.58 4.6
34 Tropomyosin 1,2 4507649 7/31 23% 40 4.5 32.99 4.6
35 Cytoskeletal tropomyosin 37424 8/24 25% 32 4.5 27.97 4.8
36 Heat Shock 27 kD protein 1 4504517 6/21 32% 30 5.5 22.84 6.0
37* heterogeneous nuclear ribonucleoprotein A1 4504445 IEVIEIMTDR (Met-ox)   40 8.5 34.15 9.3
38* Lactate dehydrogenase B 4557032 SLADELALVDVLEDK   38 5.4 36.64 5.7
      LIAPVAEEEATVPNNK          
39* Elongation factor-1-delta 4503479 IASLEVENQSLR   35 4.6 31.22 4.6
40 Heat shock 70kD protein 1 (Mitochondrial hsp70 precursor)  5123454 6/27 14% 75 5.4 73.76 6.0

* Asterisks denote proteins identified by nano-electrospray MS/MS sequence analysis (underlined residues were determined). All other protein identifications were by peptide mass mapping using MALDI-TOF MS.