Proteins in Proliferating ARPE19 RPE Cells - Microsomal fraction (Gel 26D)

Spot # Protein NCBInr # Peptide Sequence On Gel Theoretical
    Accession # Matches Coverage % MW (kDa) pI MW (kDa) pI
                 
1 Transitional endoplasmic reticulum ATPase (Valosin-containing protein) 6005942 9/22 19% 100 5.1 89.32 5.1
2 78 kD Glucose-regulated protein (GRP-78)(BIP) 6470150 17/32 28% 75 5.0 72.12 5.0
3 Pyruvate kinase, M1 + M2 Isozymes 266427 8/22 23% 60 5.1 58.30 7.8
4 Pyruvate kinase, M1 + M2 Isozymes 266427 8/23 21% 60 5.2 58.30 7.8
5 Pyruvate kinase, M1 + M2 Isozymes 266427 9/23 28% 60 5.3 58.30 7.8
6 Protein disulfide isomerase (ER-60 protease) (GRP58) 2507461 12/32 26% 60 5.5 56.68 6.1
7 ARP3 (Actin Related Protein 3) 5031573 9/21 22% 50 5.5 47.37 5.6
8 26S Protease regulatory subunit 7 547930 13/32 34% 50 5.6 49.03 5.7
9 Tryptophanyl-tRNA Synthetase 135191 8/25 23% 55 5.8 53.51 5.8
10 T-Complex protein 1, alpha subunit 135538 10/26 29% 60 5.7 60.34 5.8
11 T-Complex protein 1, gamma subunit 1729873 8/23 18% 60 6.0 60.90 6.2
12 T-Complex protein 1, zeta subunit 730922 7/24 17% 60 6.2 58.02 6.2
13 TATA Binding interacting protein 49 KD (TIP49) 4506753 8/26 20% 50 6.2 50.56 6.0
14 Aldehyde Dehydrogenase 4502031 9/31 25% 60 6.3 54.84 6.3
15 Cytokeratin 18 4557888 12/26 34% 50 5.3 47.33 5.3
16 Actin 481515 10/34 37% 45 5.3 42.14 5.3
17 T-Complex protein 1, alpha subunit (frag) 135538 6/21 15% 40 5.6 60.86 6.0
18 Glyceraldehyde-3-phosphate dehydrogenase (GPDH) 136060 5/19 34% 40 5.4 35.92 8.6
19 Glyceraldehyde-3-phosphate dehydrogenase (GPDH) 136060 4/16 27% 40 5.5 35.92 8.6
20 Lactate dehydrogenase B 4557032 9/26 33% 35 5.5 36.79 5.7
21 Heat Shock 27kD protein 1 4504517 6/20 33% 30 5.8 22.78 6.0
22 Proteasome Component C2 (Macropain Subunit C2, proteasome nu chain) 130848 7/25 36% 30 6.2 29.56 6.2
23 Proteasome component C7-I (Macropain Subunit C7-1) 1709762 5/22 33% 20 6.5 20.16 6.5
24 alpha-Crystallin B chain 4503057 7/29 58% 20 6.3 20.16 6.8
25 alpha-Crystallin B chain 4503057 5/30 34% 20 6.3 20.16 6.8
26 alpha-Crystallin B chain 4503057 4/21 48% 20 6.6 20.16 6.8
27 alpha-Crystallin B chain 4503057 10/33 69% 20 6.8 20.16 6.8
28 Glyceraldehyde-3-phosphate dehydrogenase (GPDH) 136060 4/18 27% 40 6.8 35.92 8.6
29 Glyceraldehyde-3-phosphate dehydrogenase (GPDH) 136060 9/28 46% 40 7.5 35.92 8.6
30 Glyceraldehyde-3-phosphate dehydrogenase (GPDH) 136060 12/31 52% 40 7.9 35.92 8.6
31 Annexin II (Lipocortin II) 4757756 14/30 36% 40 7.0 38.60 7.6
32 Aldolase A 113606 13/31 52% 40 7.5 39.75 8.8
33 26S Protease regulatory subunit S10B (Proteasome Subunit P42) 2492522 11/24 33% 45 6.9 44.45 7.1
34 alpha-Enolase 4503571 13/26 36% 50 6.7 47.38 7.0
35 alpha-Enolase 4503571 10/24 27% 50 6.5 47.38 7.0
36 alpha-Enolase 4503571 9/24 32% 47 6.2 47.38 7.0
37* Transitional endoplasmic reticulum ATPase (Valosin-containing protein) 6005942 EVDIGIPDATGR   98 5.2 89.32 5.1
      GILLYGPPGTGK          
38* prolyl 4-hydroxylase beta-subunit 2507460 ENLLDFIK   65 4.7 57.12 4.8
      ILEFFGLK          
39* cytokeratin 10 547749 GSLGGGFSSGGFSGGSFSR   60 5.0 57.25 5.0
      ELTTEIDNNIEQISSYK          
40 Annexin II (Lipocortin II) 4757756 7/31 22% 40 7.4 38.6 7.6
41 Proteasome Subunit C3 130850 6/30 24% 29 6.6 25.4 6.9
42 Pryuvate Kinase, M1 isozyme 266427 7/21 19% 60 7.0 57.99 7.2
43 T-Complex protein 1, eta subunit 3041738 6/40 16% 59 7.0 59.35 7.7
44 Pyruvate kinase, M1 + M2 Isozymes 266427 7/41 23% 64 6.8 57.86 7.8
45 beta-centractin 563888 5/30 22% 47 5.9 37.08 6.2

* Asterisks denote proteins identified by nano-electrospray MS/MS sequence analysis (underlined residues were determined). All other protein identifications were by peptide mass mapping using MALDI-TOF MS.