Department of Pathobiology | Technologists
Welcome to the Pathobiology Laboratory Research Assistant Website!
This website has been developed to establish a network of peers for the purpose of sharing resources and experience for the betterment of our research.
Lab assistant.The mere title can conjure up a picture of a hunch-backed, wide-eyed assistant that goes by the name Igor - a scary-looking shadow of a person, obediently following behind the mad scientist, as he collects bones and brains and other body parts for his nefarious experiments. But that’s only in the movies.
In reality, the lab assistant, or research technician, is an important part of a scientific lab, participating in high-tech, high-profile research that, in some instances, saves lives. The research technician is the workhorse of the lab. They set up, operate, and maintain the lab equipment. They test, monitor, and keep detailed logs of the experiments. Most of the work is not glamorous, and can at times be mundane. More experienced technicians maintain complex computer equipment, interpret data, develop conclusions, and devise solutions to problems under the direction of the scientist in charge…. Research technicians are exposed to dangerous chemicals, toxic materials, and even infectious diseases on a daily basis. It takes a clear mind and a steady hand to work in a lab.
The research technician may not get all the glory of the scientist; but without them, the scientist is like a conductor without an orchestra.
| Ordering |
Alquam Mashir; Becky Sebastian |
| Purchase Record Maintenance |
Alquam Mashir; Becky Sebastian |
| Breeding/weaning |
Amanda Metzger; Lisa Ruple; Yang Yu |
| Veterinary Issues |
Amanda Metzger |
| Animal Handling |
Amanda Metzger |
| Injection/Blood Draw |
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Saphenous |
Amanda Metzger |
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Ocular |
Amanda Metzger |
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IP |
Amanda Metzger |
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IM |
Amanda Metzger |
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SQ |
Amanda Metzger |
| Tissue Harvesting |
Amanda Metzger; Lisa Ruple |
| Organ Perfusion |
Cindy Kangas |
| Surgical Procedure |
Amanda Metzger |
| Fluorophore Assisted Carbohydrate Electrophoresis (FACE) |
Hyunjin Rho |
| PAGE and Western Blotting |
Joseph Cody; Lori Mavrakis; Yingchun Han; Jennifer Leising; Alquam Mashir; Hyunjin Rho; Lisa Ruple; Susamma Abraham; Yang Yu; Becky Sebastian |
| Hyaluronic acid sizing |
Joseph Cody; Alquam Mashir; Hyunjin Rho |
| ELISA |
Alquam Mashir |
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rat MPO |
Xiaoying Tang |
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cGMP |
Xiaoying Tang |
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Hyaluronic Acid |
Xiaoying Tang,Hyunjin Rho |
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Defensin beta 2 |
Hyunjin Rho |
| IHC |
Yingchun Han; Lisa Ruple; Becky Sebastian |
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fresh frozen |
Cindy Kangas |
| paraffin-embedded |
Cindy Kangas |
| OCT frozen |
Cindy Kangas |
| insitu hybridization |
Cindy Kangas |
| protein purification |
Lori Mavrakis; Hyunjin Rho |
| FACS |
Lori Mavrakis; Jennifer Leising |
| radiolabelling |
Lori Mavrakis |
| transfection |
Lori Mavrakis; Susamma Abraham |
|
lentivirus |
Jennifer Leising |
| Column Chromatography |
Lori Mavrakis; Susamma Abraham |
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gel filtration |
Lori Mavrakis; Hyunjin Rho |
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affinity |
Hyunjin Rho |
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hydroxyapatite |
Hyunjin Rho |
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ion exchange |
Hyunjin Rho; Clarita Duenas |
| Immunoprecipitation |
Yingchun Han; Jennifer Leising |
| Enzymology |
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polymerase |
Jennifer Leising |
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Receptor binding Assay |
Hyunjin Rho |
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Radio Immune Assay |
Hyunjin Rho; Susamma Abraham |
| Nitric Oxide measurement (Seivers 280i) |
Alquam Mashir |
| protein determination |
Alquam Mashir |
| Plasma isolation |
Alquam Mashir |
| WBC isolation |
Alquam Mashir |
| RBC isolation |
Alquam Mashir |
| platelet-rich plasma isolation |
Alquam Mashir |
| platelet-poor plasma isolation |
Alquam Mashir |
| platelet lysate prep |
Alquam Mashir |
| Gas Chromatography-Mass Spectrometry (GC-MS) |
Clarita Duenas; Lourdes Gruca |
| HPLC |
Clarita Duenas; Lourdes Gruca |
| BioPlex (Luminex) Multiphlex Assays |
Carole Bennett |
| Smooth muscle cell isolation from human colon |
Hyunjin Rho |
| monocyte adhesion assy |
Hyunjin Rho |
| Zymography |
Lisa Ruple; Yang Yu |
| Cutting paraffin and frozen tissues |
Yang Yu |
| Laser Microdissection |
Lisa Ruple |
| General technique |
Lori Mavrakis; Alquam Mashir; Hyunjin Rho |
| 19 Lu |
Jennifer Leising |
| 293T |
Jennifer Leising; Susamma Abraham |
| 3T3 |
Jennifer Leising |
| 3T3-L1 |
Becky Sebastian |
| A549 |
Joseph Cody; Susamma Abraham |
| C2C12 |
Yang Yu |
| HCC1937 |
Joseph Cody |
| HEK |
Jennifer Leising |
| Hela |
Susamma Abraham |
| Hep G2 |
Yang Yu |
| human mast cell |
Yingchun Han |
| NRP-152/154 |
Jennifer Leising |
| PASMCs |
Joseph Cody |
| RAW 264.7 |
Susamma Abraham |
| SK-BR-3 |
Joseph Cody |
| TLp-1 |
Susamma Abraham |
| U937 |
Joseph Cody |
| Patient consent |
Alquam Mashir |
| Collection of Breath Samples |
Alquam Mashir |
| SYFT-MS |
Alquam Mashir |
| Nitric Oxide measurement |
Alquam Mashir |
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NIOX-Flex |
Alquam Mashir |
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NIOX MINO |
Alquam Mashir |
| PFTs (DLNO, DLCO, FEVs) |
Alquam Mashir |
| Submission of IRB amendments |
Alquam Mashir |
| ultracentrifuge |
Lori Mavrakis; Becky Sebastian |
| luminometer |
Lori Mavrakis |
| scintillator |
Lori Mavrakis |
| luminometer |
Lori Mavrakis |
| gamma counting |
Lori Mavrakis |
| Spectrometry |
Lori Mavrakis; Clarita Duenas; Becky Sebastian |
| Kodak Image Station |
Becky Sebastian |
| Fluorimeter |
Clarita Duenas |
| Seivers 280i |
Alquam Mashir |
| SYFT-MS |
Alquam Mashir |
| NIOX-Flex |
Alquam Mashir |
| Gas Chromatography-Mass Spectrometry (GC-MS) |
Clarita Duenas; Lourdes Gruca |
| HPLC |
Clarita Duenas; Carole Bennett |
| BioPlex (Luminex) Multiphlex Assays |
Carole Bennett |
| Cutting paraffin and frozen tissues |
Yang Yu |
| Laser Microdissection |
Lisa Ruple |
| lyophilizer |
Lori Mavrakis |
| Phosphorimeter |
Lori Mavrakis |
| Mili Q Water |
Lori Mavrakis |
| RNA isolation |
Joseph Cody; Hyunjin Rho; Yang Yu |
| DNA isolation |
Hyunjin Rho; Lisa Ruple; Nancy Rebert |
| cDNA synthesis |
Joseph Cody; Yang Yu |
| Subcloning |
Hyunjin Rho |
| Real-Time PCR |
Xiaoying Tang; Hyunjin Rho; Susamma Abraham; Yang Yu; Nancy Rebert |
| Reverse-Transcription PCR |
Yingchun Han; Hyunjin Rho; Susamma Abraham |
| PCR |
Joseph Cody; Yingchun Han; Jennifer Leising; Hyunjin Rho; Lisa Ruple; Susamma Abraham; Yang Yu |
| DNA sequencing |
Lori Mavrakis; Susamma Abraham |
| Northern Blotting |
Lori Mavrakis; Hyunjin Rho |
| Gene Silencing
shRNA Jennifer Leising |
| Cloning |
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transformation |
Jennifer Leising; Hyunjin Rho; Susamma Abraham |
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plasmid isolation |
Jennifer Leising; Hyunjin Rho |
| single strand conformational polymorphism |
Hyunjin Rho |
| yeast II hybridization |
Hyunjin Rho |
| Primer Design |
Hyunjin Rho |
| Data analysis of DNA sequence |
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| Genotyping |
Lisa Ruple; Susamma Abraham; Yang Yu |
| | Single nucleotide polymorphism (SNP) |
Nancy Rebert |
| EMSA |
Susamma Abraham |
| Luciferase Assays |
Susamma Abraham |
| Image J |
Becky Sebastian |
| JMP statistical software |
Alquam Mashir |
| SAS |
Becky Sebastian |
| End Note |
Alquam Mashir |
| Procite |
Becky Sebastian |
| Gel Pro Analyzer |
Hyunjin Rho |
| Canvas |
Hyunjin Rho |
| Graph Pad Prism |
Hyunjin Rho; Becky Sebastian |