Research Core Services
Cell Culture Core
Cell Culture Training
The Core provides training on good lab practice to new researchers. The training can be tailored to individual needs and includes aseptic technique and culturing and maintaining cell lines.
The Core can accommodate the storage of cryovials. The core offers this service to LRI researchers for backup storage of precious cell lines.
Direct Mycoplasma Testing
This method uses an enriched agar to support the growth of colonies. Cells and supernatant are swabbed onto agar and incubated in a modular incubator chamber. Samples are viewed microscopically every other day for 2 weeks. Mycoplasma contamination is detected by the appearance of a fried egg -like growth.
EBV Transformations/Separation of Whole Blood
The Cell Core can provide immortalization of lymphocytes with the Epstein Barr Virus (EBV). The core will separate the whole blood, collect the lymphocytes, infect with EBV, and establish a cell line.
Eradication of Mycoplasma from Cell Cultures
The core will test for and eradicate mycoplasma from your cells. If mycoplasma contamination is present in your cultures the core will begin to eradicate using specialized antibiotics. This will take several weeks to complete. At the end of the treatment the core will provide clean cells growing in culture as well as several frozen vials.
Indirect Mycoplasma Testing
The core also uses a quick method to detect mycoplasma. This kit detects the four most common types of mycoplasma to contaminate cells. This is useful in determining the type of mycoplasma present.
Insect Cell Culture
The Core is equipped with a 27C incubator that is an optimum temperature to support the growth of insect cells.
The core routinely performs a direct and an indirect method of testing for Mycoplasma. The following 2 tests are done in parallel.
Preparing your Samples for Mycroplasma Testing
The cells should be grown without antibiotics for 3-4 days. Collect cells for testing by scraping the adherent cells and collecting about 5mls of cells and media. For suspension cells, grow without antibiotics and supply 5mls for testing.
Roller Bottle Cultures
The Core has the capability of producing large scale adherent cells cultured in either 850 cm or 1750 cm roller bottles grown on a roller apparatus in a 37C warm room. This is a very suitable method when large amounts of cells are required. This method also works well with suspension cells.
The core screens FBS from different sources for optimum cell growth. The batch that proves most compatible to a broad range of cell types is provided for LRI researchers.
Spinner Cultures Cells
Useful for the production of large volumes of suspension cells. Vessels come in various sizes that are used in conjunction with a magnetic stirrer spinner base. The core can provide volumes from 100mls-10L.
The Core offers sterility services using in-house prepared broths along with regular mycoplasma and endotoxin testing. Quarterly testing results are available.