Digital Imaging Microscopy Core

Resources

Confocal Microscopes

Two Leica TCS-SP and SP-AOBS upright confocal microscopes

  • Four lasers for excitation at 351, 364, 458, 476, 488, 514, 561, and 633nm wavelengths
  • Three PMT detectors
  • Spectrophotometer (detects light from 350-800 nm in 5 nm increments)
  • Transmitted light detector
  • Leica Confocal Software

Image Processing and Analysis

Computer Workstations (others also available in the Core)
Software available:

  • Volocity, PerkinElmer
  • Metamorph, Molecular Devices
  • ImagePro Plus, Media Cybernetics
  • Leica Confocal Software, Leica Microsystems
  • Leica LAS-AF, Leica Microsystems
  • Photoshop, Adobe

Laser Microdissection (Laser Capture)

Leica AS-LMD microscope (brightfield and fluorescence)

  • Nitrogen Laser with 337nm output
  • Full range of objectives from 10X to 100X
  • Hitachi HV-C20A 3-chip color CCD camera

Multi-Photon Microscope

Leica TCS SP5 II Confocal/Multi-Photon high speed upright microscope with AOBS

  • Five lasers for excitation at 405, 458, 476, 488, 496, 514, 561, 594, and 633 nm wavelengths
  • Coherent Chameleon Vision II Ti:Sapphire MP laser with OPO for 680nm-1300nm excitation range
  • 5 spectral detectors (4 Hybrid and 1 PMT)
  • Transmitted light Dodt contrast detector
  • 4 channel reflected light NDD detectors
  • 2 channel transmitted light NDD detectors
  • Tandem scanning system (Conventional - 1line per sec/Resonant - 16,000 lines/sec)
  • 20X, 25X and 40X water immersion lenses with long working distance
  • Objective piezo for fast z-stack acquisitions

Odyssey Infrared Scanner

LI-COR Odyssey Infrared Scanning System

  • Solid-state diode lasers (680 nm and 780 nm)
  • Silicon avalanche photodiode detectors
  • Li-Cor Image Studio software (Windows and Mac) - supplied to labs
  • Searchlight Array Analyst Software (Endogen) available

Spinning Disk Confocal Microscopes

PerkinElmer UltraVox spinning disk confocal microscope

  • Six lasers for excitation at 405, 445, 488, 514, 561, and 640 nm wavelengths
  • Two Hamamatsu EMCCD cameras (512 x 512 C9100-13 and 1024 x1024 C9100-50)
  • Photokinesis (FRAP) capability
  • XYZ Piezo stage for rapid z-stack acquisition
  • Environmental chamber
  • Volocity Visualization, Restoration, and Analysis Software

Confocal microscopy can also be done on the multi-photon microscope

Stereomicroscope

Leica MZ16FA stereomicroscope (fluorescence, brightfield, darkfield)

  • Motorized zoom and focus
  • QImaging Retiga cooled CCD cameras
  • Image ProPlus acquisition software.

Slide Scanner

Leica SCN400

  • Brightfield and fluorescence
  • 5x, 10x, 20x, 40x magnification
  • Standard (1 x 3"), double, and "jumbo" slides
  • 0.25 um/pixel resolution at 40X
  • Dynamic focusing/Autofocus
  • 4 slides in a carrier scanned automatically
  • 384 slide autoloader - coming soon!

Slide Scanner

PathScan Enabler IV Microscope Slide Scanner

  • 7200 dpi resolution, 3.5 microns/pixel
  • Accepts two 1 x 3" slides
  • Image quality of 5x objective

Time-Lapse (Live) and TIRF Microscopes

Leica DMI6000 inverted microscope (fluorescence, DIC, phase contrast)

  • Motorized stage
  • Environmental chamber
  • Dual Hamamatsu cameras (ImageEM and Orca-R2)
  • Full range of Chroma filters

Time-Lapse (Live) and TIRF Microscopes

Leica AM TIRF inverted microscope (fluorescence, DIC, phase contrast, TIRF)

  • Motorized stage
  • Environmental chamber
  • Dual Hamamatsu cameras (ImageEM and Orca-R2)
  • Full range of Chroma filters
  • 405nm, 488nm, 561nm, and 633nm lasers

Wide-Field Microscopes

Four Leica DM upright microscopes (fluorescence, brightfield, phase contrast, DIC, darkfield)

  • QImaging Retiga cooled CCD cameras
  • Image ProPlus acquisition software.
  • Full range of Chroma filters

Wide-Field Microscopes

Two Leica DMIRB inverted microscopes (fluorescence, brightfield, phase contrast, DIC)

  • QImaging Retiga cooled CCD cameras
  • Image ProPlus acquisition software.
  • Full range of Chroma filters
  • Hoffman modulation contrast optics available