Illumina Infinium Whole Genome Genotyping Assay
Remember: High quality DNA equals successful genotyping.
The Genomics Core is equipped with an Illumina Beadstation and employs experienced, well-trained personnel to handle Illumina Infinium Whole Genome Genotyping (WGG) projects. However, successful genotyping studies start before DNA samples are submitted to the Core: at the desk where the experiment is planned and at the bench where the DNA sample is prepared and its purity and concentration are determined: high quality DNA samples are necessary to obtain reliable genotyping results. Therefore, investigators preparing for genotyping experiments, need to do the following:
Illumina arrays are based on the ordered arraying of oligo-coated beads. In the Infinium assay each oligonucleotide (bead type) represents a specific SNP. Since each bead type is present an average of 30 times on each array, each SNP genotype call is made an average of 30 times, resulting in industry-leading accuracy, reproducibility, and call rates (all >99 %).