Lerner Research Institute News
Read about the latest advances from Lerner Research Institute scientists, including new findings, grant awards, innovations and collaborations.
An international team of researchers, including scientists from Lerner Research Institute, has uncovered new loci (chromosomal regions) associated with heart function and development.
Electrocardiograms (EKGs), which measure the heart's electrical activity, are commonly used to evaluate and diagnose cardiac health, including the heart's rhythm and blood flow. Shown as the main spike on an EKG, the time it takes for the heart ventricles to contract and expel blood (depolarization) is called the QRS interval. Prolonged QRS intervals are associated with poor cardiac events and a predictor of mortality. Previous genome-wide association studies have identified more than 20 loci associated with QRS duration.
In this study, the team of researchers—led by Yalda Jamshidi, PhD, St. George's University of London—validated these loci and, notably, uncovered rare genetic variants never before connected with QRS duration and heart function. Up to this point, the influence of rare variants has not been widely studied.
The researchers used specialized assays to detect variants with particularly low frequencies (minor allele frequency of less than 1%) in coding regions of the DNA. They identified five new genes with variants associated with lengthened QRS duration, including ADAMTS6 (A Disintegrin-Like And Metalloproteinase with Thrombospondin Type 1 Motif 6).
Timothy Mead, PhD, who is co-first author on the paper published in Genome Biology, and Suneel Apte, MBBS, DPhil, both of Lerner's Department of Biomedical Engineering, offered their cell culture expertise and preclinical models, as well as their familiarity with the related ADAMTS9 gene, to validate this data and characterize the biological mechanism responsible for ADAMTS6's causative effect on elongated QRS intervals.
Together, the team found in cellular experiments that the genetic anomaly caused reduced secretion of the ADAMTS6 protein. Additionally, they showed in a preclinical model that having only one functional copy of the ADAMTS6 gene causes a decrease in concentration of the molecule connexin 43 (Cx43). Cx43 is a gap junction protein that helps connect neighboring heart cells, serving as the conduit that transmits electrical signals across the heart. This reduction in Cx43 likely explains the abnormal EKG patterns observed—with fewer Cx43 proteins to lubricate and propagate electrical signals, it takes longer for the ventricles to depolarize.
The other genes with damaging variants found include CSRP3, FHOD3, ISM1 and TBX5.
While additional research will be important to validate the gene associations identified in this study and to elucidate the mechanisms through which they affect cardiac development and dysfunction, this is a meaningful step forward in the quest for improved diagnosis of genetic heart conditions.