Research Core Services
Immunohistochemistry (IHC) allows investigators to visualize the expression levels and distribution of specific cell or tissue antigens. Due to the variability among antibodies and techniques, IHC requires significant knowledge of basic histology, available immunological reagents, antigen retrieval techniques and immunohistochemical procedures. It can be time consuming and may require considerable titration and optimization of antibodies before research samples can be stained. It may also require experimentation with different antigen retrieval methods for paraffin tissues as well as experimentation with different fixatives for fresh frozen tissues.