Research Core Services
Proteomics and Metabolomics Laboratory
The Proteomics and Metabolomics Core is Directed by Belinda Willard, Ph.D an expert in the use of mass spectrometry in the study of biological systems. Dr. Willard has directed the Lerner Research Institute Proteomics Core laboratory since 2008 and become involved in the Metabolomics core in 2013 and is highly experienced in the operation of multiple mass spectrometry systems including high-resolution magnetic sector instruments, tandem quadrupole instruments, ion trap instruments, and time-of-flight instruments. The core is dedicated to high-sensitivity protein sequencing and identification experiments including post-translational modification analysis and the quantitative analysis of small molecules. The goal of the core is to help investigators with the analyses of proteins and small molecule compounds of interests in biological materials using the liquid chromatography on-line tandem mass spectrometry (LC/MS/MS).
Major equipment in the core includes four liquid chromatography tandem mass spectrometry systems. Two of these, the Quattro Ultima electrospray triple quadrupole mass spectrometer and the ABSciex Triple TOF 5600 are dedicated to the analysis of small molecules. The high resolution ThermoScientific Orbitrap Elite and LTQ ion trap instruments are dedicated to Proteomic Investigations. In addition, the core also has several copies of Masslynx, MarkerView, MultiQuan, Sequest, Mascot, Sieve, and Scaffold available to aid in data analysis.