Molecular Screening Core
The Molecular Screening Core provides the use of high throughput screening (HTS) technology, as well as expertise and reagents, necessary for the screening of the library of small molecules for biologically active compounds in a variety of readout systems. The Core can provide help with the adjustment of readout systems for both cell-based and biochemical assays and establishment of screening conditions based on a pilot small-scale screening. If successful, the established system is used for a full-scale library screening in 96 well format followed by generation of the database with the results.
High throughput technology is not limited to drug discovery but can also be used by any researcher to quickly conduct hundreds of thousands of biochemical, genetic or pharmacological tests.
The Core is available for researchers at Cleveland Clinic and other biomedical research centers in the Cleveland area.
- Design & adjustment of readout systems
- Set-up and optimization of screening conditions and protocols
- Screening of diversified libraries of chemicals
- Data processing
- Assistance in designing program for chemically optimized lead compounds
Using the Synergy4 multi-detection multi-plate reader
- General requirements
- Data processing
- shRNA bank
- Stage I: establishment of the readout assay
- Stage II: primary screening
- Stage IV: optimizing hits, SAR
Forms & Pricing
shRNA Order Forms:
The Molecule Screening Core (MSC) has negotiated an opportunity to order shRNA clones from Sigma (in the form of glycerol stocks) for Lerner Research Institute researchers at a very low price on a per clone basis.
To order shRNA clones:
- Check our MSC shRNA collection of available clones with controls: LRI shRNA CLONE LIST
- If your clones are available, please download and complete the Old shRNA Order Form and email to shRNA@ccf.org
- If clones are not available please visit the Sigma website
- Download and complete the New shRNA Order Form and email to shRNA@ccf.org
- To order Lentivirus, visit the Sigma website and complete the shRNA Lentivirus Order Form
Orders from the MSC shRNA collection are available in 1-3 days; orders for new shRNA are placed on the 15th and the last day of each month and are available in ~ 2 weeks after the placement.
Please visit our page on iLab to view pricing information.
EquipmentMultidrop 384 microplate dispensers (ThermoLabsystems)
Rapid liquid dispensing/cell plating into 384- and 96-well plates. Minimum volume 20 µl per well. Detection modes and applications.
Synergy 4 modular microplate reader (BioTek)
Fluorescence intensity (200-800nm, 6 decades of dynamic range), Time-Resolved Fluorescence, Fluorescence Polarization, Luminescence (300-700nm), UV-Visible Absorbance (200-999nm); FRET, TR-FRET, BRET; end-point and kinetic measurements; well area scanning and spectral scanning .
Well formats: 6-1536.
Dual injectors, temperature control, variable speed shaking, Gen5 software (reader control and data analysis). The reader is equipped with Bio-Stack Microplate Stacker (BioTek), which allows automatic reading of up to 30 microplates at different time points (0-24h).
Laminar Flow Tissue Culture Hood
3 Nuaire Biological Safety Cabinets providing laminar flow and a sterile work environment for hybridoma tissue culture work.
1 Revco -70 celsius freezer and 1 -20 celsius freezer
The Molecular Screening Core (MSC) provides the researchers at Cleveland Clinicand other biomedical research centers in Cleveland area with the expertise, technology, instrumentation and reagents necessary for the screening of the library of small molecules for biologically active compounds in a variety of readout systems. MSC owns the diverse historical chemical library of 34,000 compounds manufactured by Chembridge Corporation, world recognized leader in this field. The Core can provide help with the adjustment of readout systems for both cell-based and biochemical assays and establishment of screening conditions based on a pilot small-scale screening. If successful, the established system is used for a full-scale library screening in 96-well format followed by generation of the database with the results. MSC is equipped with laminar flow hood, CO2 incubators and Multidrop 384 (Thermo/Labsystems) to assist in plating and growing cells in 96-well format. Wallac 1420 VICTOR2 multilabel reader can be used to collect the data in variety of readout systems. Additionally, MSC can provide the information about cytotoxicity of selected hits.
MSC also offers help with data processing and planning further steps in optimization of isolated chemicals, including primary chemoinformatics analysis, generation of virtual libraries of analogues and consulting on the tactics of compound characterization, including connection with outside partners.
Molecular Screening Core
Molecular Screening Lab