The Florida Research & Innovation Center (FRIC) Imaging Core provides access to advanced imaging technologies, including:
The Core also offers support for researchers and their projects beyond hardware. Our expert staff can provide consultation on experimental design and optimization, post-acquisition image processing, and support for analysis, visualization, and interpretation of data. The analysis workstation in the facility runs a full suite of analysis and visualization software packages for getting the most rigorous quantification and representation of your imaging data.
We are located throughout the FRIC. The primary core facility is located in room 104, with the near tissue culture room 102 acting as a dedicated support room, equipped with a biological safety cabinet, cell culture incubators, -20 degree freezers and refrigerators. We also offer calibration and setup support for other small-scale fluorescent microscopes in the building.
The FRIC Imaging Core has created a repository for instrument SOPs, laboratory safety manuals, and other technical specifications. The site also has helpful links and blog articles for researchers interested in incorporating quantitative imaging into their projects. Please visit the FRIC Imaging Core Connect Today page to access the Knowledgebase (Cleveland Clinic employees only).
This is an inverted fluorescent widefield microscope with a air objectives. It has a broad- spectrum fluorescent lamp as well as a transmitted light source. It is capable of performing fluorescent, brightfield, phase and differential interference contrast (DIC) imaging. It is best used for checking samples or performing simple imaging experiments. It is not capable of conducting live-cell imaging experiments as it does not have an environmental chamber.
THUNDER Imager Live Cell and 3D Cell Culture has all you need to maintain your cells at a close to natural state. This is a widefield fluorescent microscope that is ideal for observing cells in a better physiological context.
The Leica Stellaris is a true confocal point scanning system, including a White Light Laser as excitation light source, an Acousto Optical Beam Splitter (AOBS) and a highly sensitive, prism-based spectral detection design with computer controlled adjustable bandwidth for all internal detection channels. In combination with the new Power HyD detector family, STELLARIS 8 offers enhanced detection efficiency and photon counting as standard. The extended detection range up to 850 nm plus the expanded excitation range of the White Light Laser in the visible from 440 nm up to 790 nm allow the application and separation of an extended range of spectrally overlapping fluorophores (up to the NIR). The enhanced detection efficiency and the optimal match of excitation and detection enable long term gentle live cell imaging.
The IVIS Lumina X5 integrates a 1 inch CCD camera into our benchtop Lumina instrument providing a high throughput 20 x 20 cm FOV sufficient for performing pre-clinical imaging up with bioluminescence and fluorescence. Moreover, the large, independently deployed scintilla- tor facilitates X-ray acquisitions with seamless, accurate overlay onto the optical image at any field of view.
As with other IVIS Lumina systems, the X5 is equipped with 26 filters tunable to image fluorescent sources that emit from green to near-infrared.
The LI-COR Odyssey imaging system is designed to image Western Blots using fluorescent antibody labels. This instrument allows for more precise quantitative Western Blots and removes the guess-work from relying on exposure times in traditional blotting.
The Imaging Core provides several options for quantitative analysis and visualization of any data, not just imaging. The Imaging Core Director is always open to acquiring new software packages if it can help users get better quality and more rigorous results.