About Us
The Florida Research & Innovation Center Bioinformatics Services provides high throughput sequencing analyses for all internal and external collaborators. Our specialty is RNA-seq (bulk and single cell), but we also have extensive experience with other methods, including: WGS, WES, miRNA-seq, ChIP-seq, CLIP-seq and de novo assembly of transcriptomes and genomes.
We work closely with Lerner Research Institute's Genomics Core to provide pre-sequencing consultation for projects in experimental design, sample preparation considerations, read architecture and sequencing depth selection. Once the sequencing has been completed, we will review the results of the completed analyses with investigators.
Please contact us if you would like a letter of support for your upcoming grant.
Contacts
Adrian Reich, PhD
Project Staff
reicha@ccf.org
Dr. Reich is a bioinformatics scientist with a background in high throughput sequencing analysis over dozens of projects. He has used multiple sequencing platforms to successfully test for differential gene expression (mRNA, miRNA, and piRNA) and assemble de novo transcriptomes and genomes from single cells to whole tissues. Dr. Reich has also translated the findings from computational analyses to test for those sequences in biological samples using RNA in situ localizations and testing for proteins by mass spectrometry. Currently, he develops and maintains quality control and analysis pipelines to automatically process sequencing data produced by the genomics core at Cleveland Clinic. Dr. Reich has demonstrated a record of successful projects, using data generated from 14 different high-throughput sequencing platforms from 7 different companies.
Services
Instructions for accessing example reports for standard analyses can be found on the FRIC Bioinformatics Connect Today page (Cleveland Clinic employees only).
Bulk RNA-seq Pipeline
Standard RNA-seq analyses are charged on a fixed price model, and can include as many or as few of the available analyses. We are constantly developing new analysis modules or tools that all future customers can benefit from. Currently the pipeline can provide the following analyses:
- Read trimming
- Read mapping
- Feature (gene) counting
- Differential gene expression
- Differential exon usage
- Differential splice expression analysis
- Gene set enrichment analysis
- IPA integration
Single Cell RNA-seq Pipeline
Single cell projects are also charged on a fixed price model. Single cell analyses are developing rapidly, and we are rapidly expanding the different analyses on offer. Currently the pipeline can provide:
- Read mapping
- Feature (gene) counting
- Cell cluster separation
- Cell cluster identification
- Differential gene expression
Custom Analyses
All analyses that do not fall under current pipelines are treated as custom work and are charged by the hour. This could include amplicon sequencing analysis, figure generation, protein motif quantification, de novo transcriptome assembly, etc. If your project fits within the expertise, we would be happy to help, or put you in contact with someone who might be able to help.