The Florida Research & Innovation Center (FRIC) Flow Cytometry Core provides state-of-the-art instrumentation and technical support. The principal assets of the Core consist of analytical equipment, cell sorting capabilities, single-cell library preparation instrumentation and an expert ASCP-certified Specialist in Cytometry. Multi-parameter flow cytometric analysis is provided by a spectral flow cytometer. The Core also provides access to instrumentation capable of single cell partitioning and generation of sequencing-ready libraries, as well as to qPCR and characterization instrumentation. The Core provides access to data analysis software and analysis expertise.
Please remember to acknowledge the Flow Cytometry Core in any research published that includes data or expertise provided by the Core.
For more information on access, policies, and more, please visit the FRIC Flow Cytometry Core Connect Today page (Cleveland Clinic employees only).
Leveraging the power of full spectral flow cytometry, our four laser Aurora Spectral Analyzer can detect 20+ parameters on each cell. Auto-fluorescence subtraction and state-of-the-art detectors allow for a much higher resolution, especially for problematic cell types with high background or low expression markers.
FACSAria Cell Sorters are available for the physical separation of cell populations identified via flow cytometry. FACSAria sorters allow for the detection of up to 16 fluorescent parameters excited by four laser lines, as well as forward scattered and side scattered laser light. They allow sorting of up to four populations at a time, with deposition into a variety of tube types, or deposition of one population at a time into/onto well plates (up to 384) or slides.
10x Chromium Controllers are available for the generation of single cell sequencing libraries. This powerful technology supports kits that include single cell gene expression and immune profiling, as well as ATAC and/or protein expression multiomics. After preparation of sequencing libraries, the Core can help coordinate sequencing offsite with Lerner Research Institute's Genomics Core and the FRIC Bioinformatics Core can assist with experiment design and data processing.
Assistance is available with experimental design, cell preparation troubleshooting, assay and application selection, preparation of data or methods for publication, and more. The Director of the Flow Cytometry Core is an ASCP-certified Specialist in Cytometry with over 16 years of experience in flow cytometry.
Training on instrumentation, techniques and data analysis tools is currently offered as needed. Get trained on the Aurora spectral flow cytometer to utilize it self-serve 24/7. Training on data analysis software, including FlowJo, FCSExpress, FACSDiva and more, is available, though for basic introductory training, please see the vendor websites first.
For service rates and scheduling, please visit our iLab page.
For more information on services and instrumentation, please visit the FRIC Flow Cytometry Connect Today page (Cleveland Clinic employees only).
A spectral flow cytometer capable of 20+ parameter detection. Features include:
Cell sorters capable of flow cytometric analysis and target cell (or particle) purification. Features include:
Partitions single cells for downstream library preparation. Applications include: