The Microbial Sequencing & Analytics Resource (MSAAR) facility provides investigators with a single point of contact for study design, power calculations, automated nucleic acid (DNA/RNA) extraction, sequencing library preparation and integrative microbiome informatics and analysis of the sequencing data. Our facility is equipped with the renowned epMotion® 5075tc liquid handling framework (n=2), which are fully automated and optimal for high-throughput DNA/RNA extraction and sequencing library preparation of microbiome samples. These liquid handling frameworks are optimal for nucleic acid extraction from high (e.g. stool) to low microbial biomass samples (e.g. tissue.). Other services include qPCR based absolute abundance quantification (species/strain specific) of microbial taxa and DNA/RNA quality control on an Agilent Technologies Bioanalyzer.
The facility also offers a full range of microbiome based multi-omic informatics services. We have high-performance computing nodes (746 GB RAM each) dedicated to to analyzing sequencing data from microbial genomics (clonal isolates), amplicon (16S/18S rRNA gene and internal transcribed spacer DNA), metagenomics (DNA from microbial community), metatranscriptomics, that is studies of the human microbiome as well as metabolomic analysis of the human microbiota. The analytics division has extensive experience with large (e.g. longitudinal) metagenomic and metatranscriptomic projects. We provide investigators with custom designed tools to integrate data from microbial genomics, metagenomics, metatranscriptomics and metabolomics projects and interpret metabolic activities of uncultivable microbiota and assess its impact on the host.
The Microbial Sequencing & Analytics Resource (MSAAR) is equipped with an epMotion® 7575tc liquid handling framework, assuring dependable and reproducible assay results. These instruments are purposed solely for microbiome nucleic extraction and library preparation of microbiome samples.
The MSAAR facility amplifies & prepares Illumina libraries for the following applications:
All samples in the MSAAR facility undergo quality control (QC) procedures, ensuring the quantity and the quality of the nucleic acids and prepared libraries are fit to move forward in preparation for NGS (Next Generation Sequencing). Our QC services are also available to any investigator within the Cleveland Clinic and its affiliates.
The Microbial Sequencing & Analytics Resource (MSAAR) is equipped with illumina’s iSeq™ 100 Sequencing System for affordable, fast and efficient low-throughput sequencing. This instrument has a maximum output of 4 million reads, accommodating investigators who would like to:
The Microbial Sequencing & Analytics Resource (MSAAR) provides investigators with an end-to-end resource from study design, nucleic acid extraction, NGS library preparation and comprehensive microbiome based multi-omic informatics. The MSAAR facility is conveniently housed on the fifth floor of the NE building on the Lerner Research Institute main campus. The facility includes a wet lab, freezer space, 2 high-performance computing nodes (746 GB RAM each) for amplicon and shot-gun microbiome DNA/RNA extraction and NGS library preparation.
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