Kewal Asosingh Laboratory

Research

• Overall areas of interest 
  • Airway inflammation, lung vascular biology, regional airflow

• Tools and expertise of the lab (with references)
  • Endothelial Colony-Forming Cell (ECFC) assays (Duong Pulm Circ 2011, Asosingh Lung Stem Cells 2015)
  • Bone marrow-derived proangiogenic hematopoietic progenitor assays (Asosingh JCI 2018 JI 2016 JACI 2010)
  • Asthma models  and airway hyperreactivity quantification (Asosingh JCI 2018, Ghosh PNAS 2016, Kim Immunity 2020)
  • 4Dx regional airflow imaging of lung function (Thurgood J Synchrotron Radiat 2016, Murrie Sci Rep 2020)
  • Pulmonary Arterial Hypertension models and Right ventricular hemodynamic measurements (Asosingh Blood Adv. 2017 Blood 2012)
  • Mitochondria tracing models (Stephens Mitochondrion 2020)
  • Flow Cytometry and Single-Cell Omics (Reichard Cytometry A. 2019 Cytometry A. 2018, Saygin Cytometry A. 2018)

• Examples of collaborators inside and outside of I&I
  • Xioaxio Li, Dennis Stuehr, Serpil Erzurum/ Asthma Inflammation Research
  • Serpil Erzurum/Nitric Oxide in Pulmonary Hypertension
  • Arnab Ghosh/ sGC Dysfunction in Asthma
  • Raed Dweik/ Mechanisms of Metabolic Dysregulation in Pulmonary Hypertension

• Lab discoveries
  • Discovered vascular mechanisms in allergic airway inflammation and remodeling in asthma (Asosingh JCI Insight. 2020 JCI 2018 J Immunol. 2016 J Immunol. 2013)
  • Discovered bone marrow contribution to the development of vascular disease in PAH (Asosingh Blood Adv. 2017 Blood 2012 Am J Pathol 2008, Farha Blood 2011, Duong Pulm Circ. 2011)
  • Developed various methods and best practices in Flow Cytometry (Wanner Methods Mol Biol 2019, Reichard Cytometry A. 2019 Cytometry A. 2018, Saygin Cytometry A. 2018)

• How could these discoveries help patients?
  • New therapies for asthma and pulmonary arterial hypertension
  • Better understanding of disease mechanisms.

Selected Publications

1: Rose JA, Wanner N, Cheong HI, Queisser K, Barrett P, Park M, Hite C, Naga

Prasad SV, Erzurum S, Asosingh K. Flow Cytometric Quantification of Peripheral

Blood Cell β-Adrenergic Receptor Density and Urinary Endothelial Cell-Derived

Microparticles in Pulmonary Arterial Hypertension. PLoS One. 2016 Jun

7;11(6):e0156940. doi: 10.1371/journal.pone.0156940. eCollection 2016. PubMed

PMID: 27270458; PubMed Central PMCID: PMC4896479.

2: Xu W, Ghosh S, Comhair SA, Asosingh K, Janocha AJ, Mavrakis DA, Bennett CD,

Gruca LL, Graham BB, Queisser KA, Kao CC, Wedes SH, Petrich JM, Tuder RM, Kalhan

SC, Erzurum SC. Increased mitochondrial arginine metabolism supports

bioenergetics in asthma. J Clin Invest. 2016 May 23. pii: 82925. doi:

10.1172/JCI82925. [Epub ahead of print] PubMed PMID: 27214549.

3: Ghosh A, Koziol-White CJ, Asosingh K, Cheng G, Ruple L, Groneberg D, Friebe A,

Comhair SA, Stasch JP, Panettieri RA Jr, Aronica MA, Erzurum SC, Stuehr DJ.

Soluble guanylate cyclase as an alternative target for bronchodilator therapy in

asthma. Proc Natl Acad Sci U S A. 2016 Apr 26;113(17):E2355-62. doi:

10.1073/pnas.1524398113. Epub 2016 Apr 11. PubMed PMID: 27071111; PubMed Central

PMCID: PMC4855555.

4: Asosingh K, Vasanji A, Tipton A, Queisser K, Wanner N, Janocha A, Grandon D,

Anand-Apte B, Rothenberg ME, Dweik R, Erzurum SC. Eotaxin-Rich Proangiogenic

Hematopoietic Progenitor Cells and CCR3+ Endothelium in the Atopic Asthmatic

Response. J Immunol. 2016 Mar 1;196(5):2377-87. doi: 10.4049/jimmunol.1500770.

Epub 2016 Jan 25. PubMed PMID: 26810221; PubMed Central PMCID: PMC4761512.

5: Gupta MK, Asosingh K, Aronica M, Comhair S, Cao G, Erzurum S, Panettieri RA

Jr, Naga Prasad SV. Defective Resensitization in Human Airway Smooth Muscle Cells

Evokes β-Adrenergic Receptor Dysfunction in Severe Asthma. PLoS One. 2015 May

29;10(5):e0125803. doi: 10.1371/journal.pone.0125803. eCollection 2015. PubMed

PMID: 26023787; PubMed Central PMCID: PMC4449172.

6: Barnes JW, Tian L, Heresi GA, Farver CF, Asosingh K, Comhair SA, Aulak KS,

Dweik RA. O-linked β-N-acetylglucosamine transferase directs cell proliferation

in idiopathic pulmonary arterial hypertension. Circulation. 2015 Apr

7;131(14):1260-8. doi: 10.1161/CIRCULATIONAHA.114.013878. Epub 2015 Feb 6. PubMed

PMID: 25663381; PubMed Central PMCID: PMC4390469.

7: Roach EC, Park MM, Tang WH, Thomas JD, Asosingh K, Kalaycio M, Erzurum SC,

Farha S. Impaired right ventricular-pulmonary vascular function in

myeloproliferative neoplasms. J Heart Lung Transplant. 2015 Mar;34(3):390-4. doi:

10.1016/j.healun.2014.09.009. Epub 2014 Sep 28. PubMed PMID: 25638299; PubMed

Central PMCID: PMC4377111.

8: Farha S, Dweik R, Rahaghi F, Benza R, Hassoun P, Frantz R, Torres F, Quinn DA,

Comhair S, Erzurum S, Asosingh K. Imatinib in pulmonary arterial hypertension:

c-Kit inhibition. Pulm Circ. 2014 Sep;4(3):452-5. doi: 10.1086/677359. PubMed

PMID: 25621158; PubMed Central PMCID: PMC4278604.

9: Rose JA, Erzurum S, Asosingh K. Biology and flow cytometry of proangiogenic

hematopoietic progenitors cells. Cytometry A. 2015 Jan;87(1):5-19. doi:

10.1002/cyto.a.22596. Epub 2014 Nov 21. Review. PubMed PMID: 25418030; PubMed

Central PMCID: PMC4356492.

10: Lanzola E, Farha S, Erzurum SC, Asosingh K. Bone marrow-derived vascular

modulatory cells in pulmonary arterial hypertension. Pulm Circ. 2013

Dec;3(4):781-91. doi: 10.1086/674769. Review. PubMed PMID: 25006394; PubMed

Central PMCID: PMC4070843.

11: Ratanamaneechat S, Neumann DR, Difilippo FP, Comhair SA, Asosingh K, Ghosh S,

Grandon D, Khan A, Aronica MA, Erzurum SC. Redox imaging of inflammation in

asthma. Am J Respir Crit Care Med. 2014 Mar 15;189(6):743-6. doi:

10.1164/rccm.201310-1872LE. PubMed PMID: 24628314; PubMed Central PMCID:

PMC3983839.