Jack Crabb

Computer Analyst

Department of Ophthalmic Research, Cole Eye Institute

Telephone: (216) 445-0424

Fax : (216) 445-3670

Email: crabbj1@ccf.org

ACADEMIC AND PROFESSIONAL TRAINING:

A.A.	2001, Lakeland Community College, Kirtland, OH - MicroComputer Client/Server

B.A.	2004, Case Western Reserve University, Cleveland, OH - Computer Science

Professional Experience:

2006-present	Computer Analyst, Department of Ophthalmic Research. The Cleveland Clinic Foundation, Cleveland, OH
2004-2006	Research Technologist, Department of Ophthalmic Research. The Cleveland Clinic Foundation, Cleveland, OH
1999-2004	Laboratory Assistant, Department of Ophthalmic Research. The Cleveland Clinic Foundation, Cleveland, OH

MEMBERSHIPS:

Association for Research in Vision and Ophthalmology (ARVO);

American Society for Mass Spectrometry (ASMS).

PUBLICATIONS:

Zhan X, Du Y, Crabb JS, Gu X, Kern TS, Crabb JW. (2008) Targets of tyrosine nitration in diabetic rat retina. Mol Cell Proteomics 7(5):864-74

Ng KP, Gugiu B, Renganathan K, Davies MW, Gu X, Crabb JS, Kim SR, Rózanowska MB, Bonilha VL, Rayborn ME, Salomon RG, Sparrow JR, Boulton ME, Hollyfield JG, Crabb JW. (2008) Retinal pigment epithelium lipofuscin proteomics. Mol Cell Proteomics 7(7):1397-405.

Bhattacharya SK , Crabb JS, Bonilha VL, Gu X, Takahara H, and Crabb JW (2006) Proteomics Implicates Peptidyl Arginine Deiminase 2 and Optic Nerve Citrullination in Glaucoma Pathogenesis. Invest Ophthalmol Vis Sci. 47(6):2508-14.

Bonilha VL, ME Rayborn , SK Bhattacharya , X Gu, JS Crabb, JW Crabb, and JG Hollyfield (2006) "The Retinal Pigment Epithelium Apical Microvilli And Retinal Function" In : RETINAL DEGENERATIVE DISEASES (JG Hollyfield, and RE Anderson, MM LaVail, eds), Springer, New York (pp519-524).

Bhattacharya SK, JS Crabb, SP Annangudi, KA West, X Gu, J Sun, VL Bonilha, G Smejkal, K Shadrach, JG Hollyfield and JW Crabb (2006) "Optic Nerve Fractionation for Proteomics" In: SEPARATION METHODS IN PROTEOMICS (GB Smejkal and A Lazarev, eds), CRC Taylor & Francis, Boca Raton, FL (pp 135-156).

Bhattacharya SK , KA West, X Gu, JS Crabb, K Renganathan, Z Wu, J Sun and JW Crabb (2006) "Fractionation of Retina for Proteomic Analyses" In: SEPARATION METHODS IN PROTEOMICS (GB Smejkal and A Lazarev, eds), CRC Taylor & Francis, Boca Raton , FL (pp 157-186).

Bhattacharya SK , EJ Rockwood, SD Smith, VL Bonilha, JS Crabb, RW Kuchtey, NG Robertson, NS Peachey, CC Morton and JW Crabb (2005) Proteomics Reveals Cochlin Deposits Associated With Glaucomatous Trabecular Meshwork. J Biol Chem 280(7):6080-4.

Bonilha VL, SK Bhattacharya, KA West, JS Crabb, J Sun, ME Rayborn, M Nawrot, JC Saari and JW Crabb (2004) Support For a Proposed Retinoid-Processing Protein Complex In Apical Retinal Pigment Epithelium. Exp Eye Res 79: 419-422.

West KA, Yan L, Shadrach K, Sun J, Hasan A, Miyagi M, Crabb JS, Hollyfield JG, Marmorstein AD, and Crabb JW. (2003) Protein Database, Human Retinal Pigment Epithelium. Molecular and Cellular Proteomics. 2(1):37-49.

Sakaguchi H, Miyagi M, Darrow RM, Crabb JS, Hollyfield JG, Organisciak DT and Crabb JW. (2003) Intense Light Exposure Changes the Crystallin Content in Retina. Exp Eye Res 76, 131-133.

West KA, Lin Y, Miyagi M, Crabb JS, Marmorstein AD, Marmorstein L and Crabb JW. (2001) A Proteome Survey of Proliferating and Differentiating Rat RPE-J cells. Exp Eye Research 73, 479-491.

ABSTRACTS:

X Gu, X Yuan, JS Crabb, K Shadrach, JG Hollyfield and JW Crabb, (2008) Quantitative Proteomic Analysis of AMD Bruch’s Membrane, Association for Research in Vision and Ophthalmology, April 27 - May 1, 2008, Fort Lauderdale, FL.

Xianglin Yuan, John S Crabb, Karen Shadrach, Xiaorong Gu, Joe G Hollyfield and John W Crabb, (2007) iTRAQ Quantitation of Proteins in AMD Bruch's Membrane Following SCX Fractionation, American Society for Mass Spectrometry, 55th ASMS Conference, June 3-7, Indianapolis, IN.

Xiaorong Gu, John S Crabb, Karen Shadrach, Xianglin Yuan, Joe G Hollyfield and John W Crabb, (2007) iTRAQ Quantitation of Proteins following SDS-PAGE Fractionation, American Society for Mass Spectrometry, 55th ASMS Conference, June 3-7, Indianapolis, IN.

John S Crabb, Xiaorong Gu, Xianglin Yuan and John W Crabb, (2007) Simple Programs for iTRAQ Bioinformatics, American Society for Mass Spectrometry, 55th ASMS Conference, June 3-7, Indianapolis, IN.

Xianglin Yuan, John S Crabb, Karen Shadrach, Xiaorong Gu, Joe G Hollyfield and John W Crabb, (2006) Quantitative Mass Spectrometric Analysis of Proteins in Bruch’s Membrane from Normal and AMD Donor Eyes, Lerner Research Institute Research Retreat, Sept 11, Geneva-on-the-Lake, OH.

Crabb JS, Gu X, Nawrot M, Saari JC, Crabb JW (2006) Quantitative Mass Spectrometric Analysis of CRALBP-Protein Interactions. Annual Meeting of the Association for Research in Vision and Ophthalmology, April 30-May 4, 2006, Ft Lauderdale, FL. Abstract 2039.

Gu J, Crabb JS, Bala E, Renganathan K, Gu X, Hagstrom SA, Lewis H, Salomon RG, Crabb JW, Cleveland AMD Study Group (2006) Peptidomic Profiling of Plasma for AMD Biomarkers. Annual Meeting of the Association for Research in Vision and Ophthalmology, April 30-May 4, 2006, Ft Lauderdale, FL. Abstract 4173.

Gu X, Crabb JS, Nawrot M, Saari JC, Crabb JW (2006) Quantitative Mass Spectrometric Analysis of Visual Cycle Protein Interactions. Annual Meeting of the American Society for Mass Spectrometry, May 28-June 1, 2006, Seattle, WA. Poster 616.

Gu X, Crabb JS, Annangudi SP, Bonilha VL, Shadrach K, Hollyfield JG, Bhattacharya SK, Crabb JW (2005) Optical Nerve Fractionation for Proteomic Analysis. Annual Meeting of the Association for Research in Vision and Ophthalmology, May 1-5, 2005, Ft Lauderdale, FL. Abstract 1255.

Bonilha VL, Rayborn ME, Gu X, Bhattacharya SK, Crabb JS, Crabb JW, Hollyfield JG (2005) Characterization of M6a in the Retinal Pigment Epithelium Microvilli. Annual Meeting of the Association for Research in Vision and Ophthalmology, May 1-5, 2005, Ft Lauderdale, FL. Abstract 3051.

Gu J, Wu Z, Crabb JS, Gu X, Hagstrom SA, Sears JE, Lewis H, Salomon RG, Crabb JW, Cleveland AMD Study Group. (2005) Plasma Peptidomics for Predicting AMD Susceptibility. Annual Meeting of the Association for Research in Vision and Ophthalmology, May 1-5, 2005, Ft Lauderdale, FL. Abstract 2998.

Bhattacharya SK, Crabb JS, Gu X, Crabb JW (2005) Proteomics Suggests Glaucoma Pathogenesis May Involve Optic Nerve Peptidylarginine Deiminase. (2005) Annual Meeting of the Association for Research in Vision and Ophthalmology, May 1-5, 2005, Ft Lauderdale, FL. Abstract 3510.

Crabb JS, Renganathan K, Gu X, West KA, Bhattacharya SK, Wu Z, Crabb JW (2005) Fractionation of Retina for Proteomic Analysis. Annual Meeting of the Association for Research in Vision and Ophthalmology, May 1-5, 2005, Ft Lauderdale, FL. Abstract 5154.

Crabb JW, Bhattacharya SK, Crabb JS, West KA, Sun J, Saari JC, Bonilha VL. (2004) Support For a Retinoid-Processing Complex In Apical RPE. Annual Meeting of the Association for Research in Vision and Ophthalmology, April 25-29, 2004, Ft Lauderdale, FL.

Kennedy BN, Bhattacharya SK, Crabb JS, Bonilha VL, Crabb JW. (2004) Preliminary In Vivo Analysis of CRALBP Transcriptional Regulation. Annual Meeting of the Association for Research in Vision and Ophthalmology, April 25-29, 2004, Ft Lauderdale, FL. Abstract 1261.

West KA, Sun J, Yan L, Shadrach K, Hasan A, Miyagi M, Crabb JS, Hollyfield JG, Marmorstein AD and Crabb JW. (2003) Human Retinal Pigment Epithelium Protein Database. ABRF 2003, Translating biology Using Proteomics and Functional Genomics, February 10-13, 2003, Denver, CO, J Biomolecular Techniques 14:   97, P170-Th.

West KA, Yan L, Miyagi M, Crabb JS, Marmorstein AD and  Crabb JW. (2001) Quantitative 2D Gel Comparison of Proliferating and Differentiating RPE-J Cells. Proceedings of the 49th American Society of Mass Spectrometry Conference on Mass Spectrometry and Allied Topics, W167

Miyagi M, West KA, Yan  L, Crabb JS, Darrow RM, Organisciak DT and Crabb JW. (2001) Protein Nitration in Rat Retina as a Result of Light Exposure. Invest Ophthalmol Vis Sci. 42, 4,1937.

Yan L, West KA,  Miyagi M, Crabb JS,  Marmorstein AD and Crabb JW. (2001) Proteome Analysis of Human Pigment Epitelium Cell Lines D407 and ARPE-19. Proceedings of the 49th American Society of Mass Spectrometry Conference on Mass Spectrometry and Allied Topics, W176.