Kewal Asosingh, Ph.D., C.Cy.

Staff Scientist
Scientific Director of Flow Cytometry

Lerner Research Institute
9500 Euclid Avenue
Cleveland, Ohio 44195
Location:NC2-133
asosink@ccf.org
Phone: (216) 444-0891



Our laboratory is focused on pre-clinical and translational collaborative science projects in asthma and pulmonary arterial hypertension (PAH). Program project and institutional core(s) offer various in vivo models of asthma and PAH. The laboratory is equipped to perform Flexivent airway hyperreactivity analysis, right ventricle systolic pressure measurements, in vivo lung electroporation for transgene expression, and MouseOx Pulse measurements including arterial oxygen saturation, heart rate and breath rate in experimental models. We provide comprehensive analysis of lung inflammation, goblet cell metaplasia, angiogenesis and airway fibrosis in asthma models. In addition, we offer quantification of endothelial remodeling, muscularization of pulmonary arterioles, right ventricle hypertrophy and fibrosis in PAH models. Collaborative flow cytometry-centric research aimed at unraveling vascular mechanisms in asthma and myeloid mechanisms in PAH is also a main focus of our laboratory. We also provide scientific oversight over the LRI Flow Cytometry Core.

In other words ...

We provide pre-clinical testing in experimental models of asthma and pulmonary arterial hypertension and perform collaborative research of how abnormal blood vessels and bone marrow stem cells contribute to lung diseases.


Mario  Alemagno BS
Mario Alemagno BS
Research Technician

Location:NC2-134
Phone:(216) 445-7174
alemagm@ccf.org
Fax:(216) 636-0104
laboratory

Kimberly  Queisser B.A.
Kimberly Queisser B.A.
Research Technician

Location:NC2-134
Phone:(216) 445-7174
queissk@ccf.org
laboratory

Eric  Stuehr
Eric Stuehr
Research Student

Location:NC2-134
Phone:(216) 445-7174
stuehre@ccf.org
laboratory

Nicholas   Wanner
Nicholas Wanner
Research Technician

Location:NC2-131
Phone:(216) 445-6625
wannern@ccf.org
Fax:(216) 636-0104
laboratory

Kelly  Weiss
Kelly Weiss
Research Technician

Location:NC2-131
Phone:(216) 445-6625
weissk@ccf.org
laboratory


Selected Publications

 

1: Rose JA, Wanner N, Cheong HI, Queisser K, Barrett P, Park M, Hite C, Naga

Prasad SV, Erzurum S, Asosingh K. Flow Cytometric Quantification of Peripheral

Blood Cell β-Adrenergic Receptor Density and Urinary Endothelial Cell-Derived

Microparticles in Pulmonary Arterial Hypertension. PLoS One. 2016 Jun

7;11(6):e0156940. doi: 10.1371/journal.pone.0156940. eCollection 2016. PubMed

PMID: 27270458; PubMed Central PMCID: PMC4896479.

 

 

2: Xu W, Ghosh S, Comhair SA, Asosingh K, Janocha AJ, Mavrakis DA, Bennett CD,

Gruca LL, Graham BB, Queisser KA, Kao CC, Wedes SH, Petrich JM, Tuder RM, Kalhan

SC, Erzurum SC. Increased mitochondrial arginine metabolism supports

bioenergetics in asthma. J Clin Invest. 2016 May 23. pii: 82925. doi:

10.1172/JCI82925. [Epub ahead of print] PubMed PMID: 27214549.

 

 

3: Ghosh A, Koziol-White CJ, Asosingh K, Cheng G, Ruple L, Groneberg D, Friebe A,

Comhair SA, Stasch JP, Panettieri RA Jr, Aronica MA, Erzurum SC, Stuehr DJ.

Soluble guanylate cyclase as an alternative target for bronchodilator therapy in

asthma. Proc Natl Acad Sci U S A. 2016 Apr 26;113(17):E2355-62. doi:

10.1073/pnas.1524398113. Epub 2016 Apr 11. PubMed PMID: 27071111; PubMed Central

PMCID: PMC4855555.

 

 

4: Asosingh K, Vasanji A, Tipton A, Queisser K, Wanner N, Janocha A, Grandon D,

Anand-Apte B, Rothenberg ME, Dweik R, Erzurum SC. Eotaxin-Rich Proangiogenic

Hematopoietic Progenitor Cells and CCR3+ Endothelium in the Atopic Asthmatic

Response. J Immunol. 2016 Mar 1;196(5):2377-87. doi: 10.4049/jimmunol.1500770.

Epub 2016 Jan 25. PubMed PMID: 26810221; PubMed Central PMCID: PMC4761512.

 

 

5: Gupta MK, Asosingh K, Aronica M, Comhair S, Cao G, Erzurum S, Panettieri RA

Jr, Naga Prasad SV. Defective Resensitization in Human Airway Smooth Muscle Cells

Evokes β-Adrenergic Receptor Dysfunction in Severe Asthma. PLoS One. 2015 May

29;10(5):e0125803. doi: 10.1371/journal.pone.0125803. eCollection 2015. PubMed

PMID: 26023787; PubMed Central PMCID: PMC4449172.

 

 

6: Barnes JW, Tian L, Heresi GA, Farver CF, Asosingh K, Comhair SA, Aulak KS,

Dweik RA. O-linked β-N-acetylglucosamine transferase directs cell proliferation

in idiopathic pulmonary arterial hypertension. Circulation. 2015 Apr

7;131(14):1260-8. doi: 10.1161/CIRCULATIONAHA.114.013878. Epub 2015 Feb 6. PubMed

PMID: 25663381; PubMed Central PMCID: PMC4390469.

 

 

7: Roach EC, Park MM, Tang WH, Thomas JD, Asosingh K, Kalaycio M, Erzurum SC,

Farha S. Impaired right ventricular-pulmonary vascular function in

myeloproliferative neoplasms. J Heart Lung Transplant. 2015 Mar;34(3):390-4. doi:

10.1016/j.healun.2014.09.009. Epub 2014 Sep 28. PubMed PMID: 25638299; PubMed

Central PMCID: PMC4377111.

 

 

8: Farha S, Dweik R, Rahaghi F, Benza R, Hassoun P, Frantz R, Torres F, Quinn DA,

Comhair S, Erzurum S, Asosingh K. Imatinib in pulmonary arterial hypertension:

c-Kit inhibition. Pulm Circ. 2014 Sep;4(3):452-5. doi: 10.1086/677359. PubMed

PMID: 25621158; PubMed Central PMCID: PMC4278604.

 

 

9: Rose JA, Erzurum S, Asosingh K. Biology and flow cytometry of proangiogenic

hematopoietic progenitors cells. Cytometry A. 2015 Jan;87(1):5-19. doi:

10.1002/cyto.a.22596. Epub 2014 Nov 21. Review. PubMed PMID: 25418030; PubMed

Central PMCID: PMC4356492.

 

 

10: Lanzola E, Farha S, Erzurum SC, Asosingh K. Bone marrow-derived vascular

modulatory cells in pulmonary arterial hypertension. Pulm Circ. 2013

Dec;3(4):781-91. doi: 10.1086/674769. Review. PubMed PMID: 25006394; PubMed

Central PMCID: PMC4070843.

 

 

11: Ratanamaneechat S, Neumann DR, Difilippo FP, Comhair SA, Asosingh K, Ghosh S,

Grandon D, Khan A, Aronica MA, Erzurum SC. Redox imaging of inflammation in

asthma. Am J Respir Crit Care Med. 2014 Mar 15;189(6):743-6. doi:

10.1164/rccm.201310-1872LE. PubMed PMID: 24628314; PubMed Central PMCID:

PMC3983839.