04/15/2019
The Microbiome of the Inflamed Aorta: What Can We Discern?
Noninfectious aortitis is usually a result of primary systemic large-vessel vasculitis (LVV) such as giant cell arteritis (GCA) or Takayasu’s arteritis (TAK).
But inflammation of the aorta may also present as “clinically isolated aortitis” (CIA), a noninfectious vasculitis restricted to the proximal thoracic aorta. About 17 percent of patients with CIA develop features of systemic disease, most often GCA.
Researchers do not yet know whether common conditions exist within CIA- and GCA-affected aortas that contribute to inflammatory aneurysms or how aortitis specimens compare with those from noninflammatory thoracic aortic aneurysms.
Recently, however, a group of investigators compared the microbial communities within the aortas of patients with CIA and GCA with microbial communities in noninflammatory aortic aneurysm controls. They also analyzed microbiomes of temporal arteries (TA) from a parallel study and compared with those from aortic aneurysms.
“Changes in the body’s microbiome might trigger inflammation that can then affect how genes express themselves,” says Alexandra Villa-Forte, MD, MPH, staff in the Department of Rheumatic and Immunologic Diseases, who presented the research at the International Vasculitis & ANCA Workshop 2019. “We know that microbes in our gut or elsewhere in the body may play a role in the development of a wide range of diseases such as inflammatory bowel disease, type 2 diabetes mellitus, obesity and cardiovascular disease. So it makes sense to look into their role in vasculitides.”
Study characteristics
The authors obtained 220 aorta samples from prospectively enrolled patients undergoing thoracic aorta aneurysm surgery and selected 49 based on ability to match for age, gender and race (12 CIA, 14 GCA and 23 noninflammatory aneurysm controls).
The researchers collected biopsies under surgically aseptic conditions. Those samples were then snap frozen (-80°C), deidentified and processed in blind fashion.
The investigators then performed taxonomic classification of bacterial sequences to the genus level and also calculated relative abundances. They used principal coordinate analysis (PCoA/DESeq2) to analyze microbiome differential abundances and used PICRUSt to predict functional profiling.
Results of comparison
Microbial beta and alpha diversity differed between the aortitis cases and the aorta controls. There were no significant differences between microbial communities in CIA and GCA.
The largest differential abundances between aortitis and noninflammatory control samples included Enterobacteriaceae, Phascolarctobacterium, Acinetobactor, Klebsiella and Prevotella.
Overall microbiomes of aortas differed significantly from those of TAs, in both control and GCA groups.
“Contrary to previous beliefs, we found that thoracic aortic aneurysms are not sterile,” says Charis Eng, MD, PhD, Chair, Genomic Medicine Institute. “That is hopefully the first step in understanding whether microbes play a role in the pathogenesis of aortitis or noninflammatory aneurysms. We saw an important clue: that the microbiome of aortitis significantly differed from that of TA in GCA.”