Sample Preparation and Staining Protocols

1. Colloidal Coomassie Blue staining for protein identification by mass spectrometry
   
   
2. Silver staining for protein identification by mass spectrometry
   
   
3. Localization of immunoreactive 2D gel spots for excision and protein identification
   
   
4. In-gel tryptic digestion for gels stained with Coomassie Blue
   
   
5. In-gel cysteine reduction and alkylation
   
   
6. In-gel tryptic digestion in a 96-well microtiter plate for Coomassie Blue stained gels
   
   
7. In-gel tryptic digestion for silver stained gels
   
   
8. In-gel tryptic digestion in 96-well microtiter plate silver stained gels