Thomas A. Hamilton, Ph.D.

Staff
Interim Chair Stem Cell Biology and Regenerative Medicine

Lerner Research Institute
9500 Euclid Avenue
Cleveland, Ohio 44195
Location:NE4-314
hamiltt@ccf.org
Phone: (216) 444-6246
Fax: (216) 444-9329



Brief Description

The diversity of the inflammatory process stems from multiple sources, including the complexity of inflammatory stimuli and cell types, the transmembrane and intracellular signaling processes that occur following stimulation, and the large number of independently regulated genes whose expression is subject to modulation during the process.

The primary objective of our research program is to define the molecular events that control the expression of inducible genes during the initiation and resolution of inflammation. An emerging component of these studies includes consideration of the process of cellular stress, also known as the unfolded protein response or endoplasmic reticulum stress. Collectively our research efforts focus on alterations in transcription and mRNA metabolism that can produce significant changes in levels of inflammatory gene products such as chemoattractant cytokines (chemokines) and mediators of cellular stress. Although the mechanisms that increase inflammatory gene expression are important, it is equally necessary to understand negative regulation of these genes since the expression patterns are transient and inappropriate or prolonged expression often results in substantial tissue injury.

The goals of current projects include: (1) identification of mRNA sequence controlling instability and stimulus-induced stabilization, (2) definition of mechanisms through which mRNA decay is achieved, and (3) characterization of the signaling pathways through which inflammatory and cellular stress pathways communicate with one another.

In other words ...

  • Evaluation of mechanisms regulating mRNA half life during inflammatory response
  • Role of upstream open reading frames in regulating cellular stress responses
  • Signal transduction pathways coupling cell stress and inflammatory response in myeloid cells


William  M. Baldwin M.D., Ph.D.
William M. Baldwin M.D., Ph.D.
Staff

Location:NB3-75
Phone:(216) 445-2384
baldwiw@ccf.org
Fax:(216) 444-8372
web site

Shyamasree  Datta Ph.D.
Shyamasree Datta Ph.D.
Research Associate

Location:NE4-300I
Phone:(216) 444-3687
dattas@ccf.org
Fax:(216) 444-9329

Robert L. Fairchild Ph.D.
Robert L. Fairchild Ph.D.
Staff

Location:NB3-59
Phone:(216) 444-3146
fairchr@ccf.org
Fax:(216) 444-8372
web site

James H. Finke Ph.D.
James H. Finke Ph.D.
Contract Staff

Location:NE4-306
Phone:(216) 444-5186
finkej@ccf.org
Fax:(216) 444-9329
web site

Brian  Gastman M.D.
Brian Gastman M.D.
Staff

Location:NE4-303
Phone:(216) 445-2103
gastmab@ccf.org
web site

Neetu  Gupta Ph.D.
Neetu Gupta Ph.D.
Associate Staff

Location:NE4-305
Phone:(216) 444-7455
guptan@ccf.org
Fax:(216) 444-9329
web site

Trine N. Jorgensen Ph.D.
Trine N. Jorgensen Ph.D.
Assistant Staff

Location:NE4-304
Phone:(216) 444-7454
jorgent@ccf.org
Fax:(216) 444-9329
web site

Xiaoxia  Li Ph.D.
Xiaoxia Li Ph.D.
Staff

Location:NE4-307
Phone:(216) 445-8706
lix@ccf.org
Fax:(216) 444-9329
web site

Feng  Lin Ph.D.
Feng Lin Ph.D.
Staff

Location:NE4-202
Phone:(216) 445-6637
linf2@ccf.org
web site

Booki  Min D.V.M., Ph.D.
Booki Min D.V.M., Ph.D.
Associate Staff

Location:NB3-77
Phone:(216) 445-3126
minb@ccf.org
web site

Miwa  Morita Ph.D.
Miwa Morita Ph.D.
Research Associate

Location:NE6-252
Phone:(216) 444-2746
moritam@ccf.org

Kurt W. Runge Ph.D.
Kurt W. Runge Ph.D.
Staff

Location:NE2-203
Phone:(216) 445-9771
rungek@ccf.org
Fax:(216) 444-0512
web site

Ganes C. Sen Ph.D.
Ganes C. Sen Ph.D.
Staff

Location:NE4-314
Phone:(216) 444-0636
seng@ccf.org
Fax:(216) 444-9329
web site

Vincent K. Tuohy Ph.D.
Vincent K. Tuohy Ph.D.
Staff

Location:NB3-76
Phone:(216) 445-9684
tuohyv@ccf.org
Fax:(216) 444-8372
web site

Chenyang  Zhao Ph.D.
Chenyang Zhao Ph.D.
Contract Staff

Location:NE4-254
Phone:(216) 444-4669
zhaoc@ccf.org
Fax:(216) 444-9329


  1. Datta, S., Novotny, M., Pavicic, P.G., Zhao, C., Herjan, T., Hartupee, J., and Hamilton, T.A., Interleukin 17 regulates CXCL1 mRNA stability via an AUUUA/Tristetraprolin independent sequence, J. Immunol. 184 1484-1491, 2010.
  2. Zhao, C., Datta, S., Mandal, P., Xu, D., and Hamilton, T., Stress sensitive reguation of IFRD1 mRNA decay is mediated by an upstream open reading frame (ORF), J. Biol. Chem. 285: 8552-8562, 2010.
  3. Hamilton, T.A., Novotny, M., Pavicic, P. J., Herjan, T., Hartupee, J., Sun, D., Zhao, C., Datta, S., Diversity in Post-Transcriptional Control of Neutrophil Chemoattractant Cytokine Gene Expression, Cytokine, 52: 116-122, 2010
  4. Wan, Y., Xiao, H.,, Affolter, J., Kim, T.W.,, Bulek, K., Chaudhur, S., Carlson, D., Hamilton, T., Mazumder, T., Stark, G.R., Thomas, J., Li, X., IL-1 receptor-associated kinase 2 is critical for LPS-mediated post-transcriptional control, J.Biol. Chem. 284: 20041 - 20051, 2009
  5. Hartupee, J., Liu, C., Novotny, M., Sun, D., Xiaoxia Li, and Thomas A. Hamilton. IL-17 Signaling for mRNA Stabilization Does Not Require TNF Receptor-Associated Factor 6, J. Immunol. 182: 1660 - 1666, 2009
  6. Datta S, Biswas R, Novotny M, Pavicic PG Jr, Herjan T, Mandal P and Hamilton TA. Tristetraprolin regulates CXCL1 (KC) mRNA stability. J. Immunol. 180(4):2545-2552, 2008.
  7. Hartupee, J., Li, X., and Hamilton, T.A., IL-1alpha -induced NFkappaB activation and chemokine mRNA stabilization diverge at IRAK1. J. Biol. Chem. 283:15689-15693 2008