What Can The MTRC Do For You?

Basic Science

The MTRC has biochemical research space with a shared storage room for freezers in the Lerner Research Institute (LRI) at the Cleveland Clinic. The space includes sterile and fume hoods, benches, sinks, autoclave, and electrical power and safety features.

SeaHorse Mitocondrial Core: We provide measures of cellular bioenergetics and mitochondrial dysfunction using the XF24 extracellular flux analyzer (Seahorse Biosciences, Billerica, MA). This equipment can measure mitochondrial respiration from live cells to intact skeletal muscle fibers isolated from tissue, cellular glycolytic function, and fat oxidation capacity.

Mass Spectrometry Core: We also have an Agilent 6890N gas chromatograph interfaced with an Agilent 5975N Mass Spectral Detector and loaded with Agilent Chemstation software for data analysis. This mass spectrometry system supports the study of whole body metabolism using stable isotope tracers, in particular glucose and amino acids.

Biochemistry Laboratory: Includes rapid determination of blood glucose for euglycemic clamp studies. The lab has three glucose analyzers (Yellow Springs), refrigerated centrifuge, fume hoods and a scintillation counter.

Molecular Biology: We can support real time RT-PCR measurements, using PX2 and Gene Amp Thermal Cyclers, MX3000P Stratagene Real Time Cyclers. The lab also has microcentrifuges, biosafety cabinets, cell culture incubators, and a large assortment of electrophoresis equipment.

Image Analysis: Includes a Kodak 400R Imaging Station, Wacom graphics tablet with tracing pad, NIH Image J analysis software, and other dedicated software packages to analyze images transferred from MRI and CT imagers, Western blots and gel images, as well as tissue sections.

Other: http://www.lerner.ccf.org/services/


Clinical research is conducted in the Clinical Research Unit (CRU) of the CTSA at the Cleveland Clinic. The CRU resources include a 7-bed research inpatient/outpatient unit providing a controlled environment for patient-oriented research. This center has unique facilities for metabolic clamps and body composition assessment.

Clinical Procedure Room: This room is designed for hyperinsulinemic-euglycemic, and hyperglycemic clamps, isotopic tracer infusion studies, muscle biopsies, indirect calorimetry, and physical examinations. Equipment used to support these procedures include Harvard PHD 2000 four-barrel and two-barrel pumps with speed modulators for the hyperinsulinemic-euglycemic clamp and Harvard infusion pumps for infusion of isotopes. In addition, a state-of-the-art Vmax Encore (Viasys Healthcare, Yorba Linda, CA) indirect calorimetry system is used for O2 and CO2 analysis, two YSI glucose analyzers (Yellow Springs, OH) for plasma glucose determination, and a quick spin microcentrifuge for plasma separation. Each room is equipped with an electric hospital bed, LCD flat-panel TV, DVD and VCR player.

Human Physiology Testing: The lab is located within the Cleveland Clinic CRU. Equipment includes an inline indirect calorimetry system (Yaeger-JEA) with breath-by-breath oxygen and carbon dioxide gas analyzers and electrocardiograph, a Lode electronically braked cycle ergometer, and a Trackmaster automatic treadmill. In addition, the lab is equipped with multiple blood pressure cuffs, automated AED and full crash cart.

Body Composition Laboratory: Housed within the Cleveland Clinic Bariatric Metabolic Institute (BMI), the lab is dedicated to the assessment of body composition and contains a Lunar iDXA unit and a whole body air displacement plethysmograph (BodPod). We can also provide waist-to-hip ratio measurements and skin caliper measurements for body fat percentage.

Metabolic Kitchen: We share a full metabolic kitchen with the CRU. The kitchen is equipped with scales and deep freezers for the preparation of dietary interventions. The metabolic kitchen is fully staffed by registered dieticians who have over 20 years of experience in research related dietary interventions.

Walker Fitness and Wellness Center: The Walker Fitness Center is a fully staffed facility that includes 4 trained and certified exercise physiology professionals. The facility offers: cardio respiratory fitness room with treadmills, cycle and arm ergometers, stair-steppers, elliptical machines, weight training room with rotary machines and free weights, full basketball court and multipurpose gym, indoor heated swimming pool, and indoor and outdoor tracks. While training, subjects are monitored for heart rate and blood pressure using telemetry devices, and are supervised by an American College of Sports Medicine certified Clinical Exercise Specialist.