Our laboratory studies nitric oxide (NO) biosynthesis in mammals. NO is made by an enzyme named NO synthase, and is involved in many aspects of cell function and disease, including signal transduction in the brain, control of blood pressure and heart rate, gastric motility, oxygen delivery, immunologic destruction of tumor cells and microbes, infertility, impotence, and stroke. We study how NO biosynthesis takes place at the molecular level and how it is regulated, and also study the impact of NO synthesis on certain aspects of cells and tissues. Our interests include the NO synthase enzyme chemistry, understanding how the enzyme’s protein structure relates to its function, and learning how other cellular proteins can interact with NO synthase to control its activity. We are also studying how NO regulates heme insertion into cellular proteins, and how protein nitration, which is a downstream consequence of NO production, takes place in cells, is regulated, and how it may control protein functions in cells and tissues.
Haque M, Panda K, Tejero J, Aulak K, Fadlalla M, Mustovich A, Stuehr DJ. A connecting hinge represses the activity of endothelial NO synthase. PNAS 2007 104:9254-9 Panda K, Haque M, Garcin-Hosfield E, Durra D, Getzoff E, Stuehr DJ. Surface charge interactions of the FMN module govern NO synthase. J Biol Chem 2006 28:36819-27 Koeck T, Stuehr DJ, Aulak K. Mitochondria and regulated tyrosine nitration. Biochem Soc Trans 2005 33:1399-403 Stuehr DJ, Wei C, Wang Z, Hille R. Exploring the redox reactions between heme and tetrahydrobiopterin in the NO synthases. Dalton Trans 2005 21:3427-35