Dennis Stuehr, Ph.D.

Dennis Stuehr, Ph.D.

Staff, Department of Pathobiology

  • Department of Pathobiology
  • Lerner Research Institute (NC22)
  • The Cleveland Clinic Foundation
  • 9500 Euclid Avenue
  • Cleveland, Ohio 44195
  • Phone: (216) 445-6950
  • Fax: (216) 444-9329
  • Fellowship - Cornell University Medical College
    New York, NY
  • Doctorate - Massachusetts Institute of Technology
    Cambridge, MA
  • Undergraduate - Bowling Green State University
    Bowling Green, OH
  • biochemistry and physiology of nitric oxide

PubMed Publications

  • Function of mitochondrial Stat3 in cellular respiration.
    Wegrzyn J, Potla R, Chwae YJ, Sepuri NB, Zhang Q, Koeck T, Derecka M, Szczepanek K, Szelag M, Gornicka A, Moh A, Moghaddas S, Chen Q, Bobbili S, Cichy J, Dulak J, Baker DP, Wolfman A, Stuehr D, Hassan MO, Fu XY, Avadhani N, Drake JI, Fawcett P, Lesnefsky EJ, Larner AC
    PubMed:19131594 :: 2009

Wang ZQ, Wei CC, Stuehr DJ. How does a valine residue that modulates heme-NO binding kinetics in inducible NO synthase regulate enzyme catalysis? J Inorg Biochem. 2010 Mar;104(3):349-356.

Guan ZW, Haque MM, Wei CC, Garcin ED, Getzoff ED, Stuehr DJ. Lys842 in neuronal nitric-oxide synthase enables the autoinhibitory insert to antagonize calmodulin binding, increase FMN shielding, and suppress interflavin electron transfer. J Biol Chem. 2010 Jan 29;285(5):3064-75.

Giroud C, Moreau M, Mattioli TA, Balland V, Boucher JL, Xu Y, Stuehr DJ, Santolini J. Role of arginine guanidinium moiety in nitric oxide synthase mechanism of oxygen activation. J Biol Chem. 2009 Nov 30.

Tejero J, Santolini J, Stuehr DJ. Fast ferrous heme-NO oxidation in nitric oxide synthases. FEBS J. 2009 Aug;276(16):4505-14.

Sabat J, Stuehr DJ, Yeh SR, Rousseau DL. Characterization of the proximal ligand in the P420 form of inducible nitric oxide synthase. J Am Chem Soc. 2009 Sep 2;131(34):12186-92.

Stuehr DJ, Tejero J, Haque MM. Structural and mechanistic aspects of flavoproteins: electron transfer through the nitric oxide synthase flavoprotein domain. FEBS J. 2009 Aug;276(15):3959-74.

Haque MM, Fadlalla MA, Wang ZQ, Sinha Ray S, Panda K, Stuehr DJ. Neutralizing a Surface Charge on the FMN Subdomain Increases the Activity of Neuronal Nitric Oxide Synthase by Enhancing the Oxygen Reactivity of the Enzyme Heme-Nitric oxide Complex. J Biol Chem. 2009 Jul 17;284(29):19237-47.

Koeck T, Corbett JA, Crabb JW, Stuehr DJ, Aulak KS. Glucose-modulated tyrosine nitration in beta cells: targets and consequences. Arch Biochem Biophys. 2009 Apr 15;484(2):221-31. PDF

Ilagan RP, Tejero J, Aulak KS, Ray SS, Hemann C, Wang ZQ, Gangoda M, Zweier JL, Stuehr DJ. Regulation of FMN subdomain interactions and function in neuronal nitric oxide synthase. Biochemistry. 2009 May 12;48(18):3864-76.

Koeck T, Willard B, Crabb JW, Kinter M, Stuehr DJ, Aulak KS. Glucose-mediated tyrosine nitration in adipocytes: targets and consequences. Free Radic Biol Med. 2009 Apr 1;46(7):884-92.

Tejero J, Biswas A, Wang ZQ, Page RC, Haque MM, Hemann C, Zweier JL, Misra S, Stuehr DJ. Stabilization and characterization of a heme-oxy reaction intermediate in inducible nitric oxide synthase. J Biol Chem. 2008 Nov 28;283(48):33498-507.

Ilagan RP, Tiso M, Konas DW, Hemann C, Durra D, Hille R, Stuehr DJ. Differences in a Conformational Equilibrium Distinguish Catalysis by the Endothelial and Neuronal Nitric-oxide Synthase Flavoproteins. J Biol Chem. 2008 Jul 11;283(28):19603-15.

Chandor A, Dijols S, Ramassamy B, Frapart Y, Mansuy D, Stuehr D, Helsby N, Boucher JL. Metabolic Activation of the Antitumor Drug 5-(Aziridin-1-yl)-2,4-Dinitrobenzamide (CB1954) by NO Synthases. Chem Res Toxicol. 2008 Apr;21(4):836-43.

Gusarov I, Starodubtseva M, Wang ZQ, McQuade L, Lippard SJ, Stuehr DJ, Nudler E. Bacterial NO-synthases operate without a dedicated redox partner. J Biol Chem. 2008 May 9;283(19):13140-7.

Wei CC, Wang ZQ, Tejero J, Yang YP, Hemann C, Hille R, Stuehr DJ. Catalytic reduction of a tetrahydrobiopterin radical within nitric oxide synthase. J Biol Chem. 2008 Apr 25;283(17):11734-42.

Tiso M, Strub A, Hesslinger C, Kenney CT, Boer R, Stuehr DJ. BYK191023 (2-[2-(4-methoxy-pyridin-2-yl)-ethyl]-3h-imidazo[4,5-b]pyridine) is an NADPH- and time-dependent irreversible inhibitor of inducible nitric-oxide synthase. Mol Pharmacol. 2008 Apr;73(4):1244-53.

Tiso M, Tejero J, Panda K, Aulak KS, Stuehr DJ. Versatile regulation of neuronal nitric oxide synthase by specific regions of its C-terminal tail. Biochemistry. 2007 Dec 18;46(50):14418-28.

Beaumont E, Lambry JC, Wang ZQ, Stuehr DJ, Martin JL, Slama-Schwok A. Distal Val346Ile mutation in inducible NO synthase promotes substrate-dependent NO confinement. Biochemistry. 2007 Nov 27;46(47):13533-40.

Erzurum SC, Ghosh S, Janocha AJ, Xu W, Bauer S, Bryan NS, Tejero J, Hemann C, Hille R, Stuehr DJ, Feelisch M, Beall CM. Higher blood flow and circulating NO products offset high-altitude hypoxia among Tibetans. Proc Natl Acad Sci U S A. 2007 Nov 6;104(45):17593-8.

Ray SS, Sengupta R, Tiso M, Haque MM, Sahoo R, Konas DW, Aulak K, Regulski M, Tully T, Stuehr DJ, Ghosh S. Reductase domain of Drosophila melanogaster nitric-oxide synthase: redox transformations, regulation, and similarity to mammalian homologues. Biochemistry. 2007 Oct 23;46(42):11865-73.

Ray SS, Tejero J, Wang ZQ, Dutta T, Bhattacharjee A, Regulski M, Tully T, Ghosh S, Stuehr DJ. Oxygenase domain of Drosophila melanogaster nitric oxide synthase: unique kinetic parameters enable a more efficient NO release. Biochemistry. 2007 Oct 23;46(42):11857-64.

Haque MM, Panda K, Tejero J, Aulak KS, Fadlalla MA, Mustovich AT, Stuehr DJ. A connecting hinge represses the activity of endothelial nitric oxide synthase. Proc Natl Acad Sci U S A. 2007 May 29;104(22):9254-9.

Li D, Kabir M, Stuehr DJ, Rousseau DL, Yeh SR. Substrate- and isoform-specific dioxygen complexes of nitric oxide synthase. J Am Chem Soc. 2007 May 30;129(21):6943-51.

Maréchal A, Mattioli TA, Stuehr DJ, Santolini J. Activation of peroxynitrite by inducible nitric-oxide synthase: a direct source of nitrative stress. J Biol Chem. 2007 May 11;282(19):14101-12.

Jagnandan D, Church JE, Banfi B, Stuehr DJ, Marrero MB, Fulton DJ. Novel mechanism of activation of NADPH oxidase 5. calcium sensitization via phosphorylation. J Biol Chem. 2007 Mar 2;282(9):6494-507.