The Flow Cytometry Core provides investigators with a resource for analytical and preparative studies of cells using flow cytometry. The Core offers FACS acquisition and analysis, cell sorting, and consultation for experimental design, interpretation and troubleshooting. Applications include immunophenotyping, intracellular staining, molecular and cellular probes such as Ca flux and GFP, CBA (Cytometric Bead Array), DNA cell cycle, TUNEL and Annexin V for apoptosis, sterile sorting for cloning and more. Both the LSRFortessa and Aria II have a green 561nm laser, which is optimal for detection of "mFruit" fluorescent proteins.
The Core is equipped with the following instruments:
The Core provides data analysis with the following software:
Analysis workstations and software training are available for researchers.
Investigators must schedule appointments in advance for ALL flow cytometry. Currently, 1-2 days notice is required; however, more notice is necessary for sorting. Viewing privileges of the appointment calendar are attainable through Google Calendar (GoogleCalendar.pdf). Please be aware calendar openings do not guarantee an appointment time. Appointments can be made by phone or e-mail. The Flow Core reserves the right to schedule as it sees fit.