Flow Cytometry Core

The Flow Cytometry Core of the Lerner Research Institute provides investigators at Cleveland Clinic with a resource for analytical and preparative studies of cells using flow cytometry. The Core offers FACS acquisition and analysis, staining for DNA cell cycle and fragmentation, and sterile cell sorting. Consultation for experimental design, interpretation and troubleshooting is also available.

Analytical instruments include a BD FACScan and a BD LSRII. The FACScan provides 3-color analysis. The BD LSRII replaced the BD LSR in June of 2007. The BD LSRII is configured with four fixed alignment lasers (488, 532, 355 and 638nm) and has the ability to detect up to 9 colors simultaneously. The new all digital electronics expands channel resolution and increases detection sensitivity. The 355nm HeCd can be used to perform Ca flux studies in cell populations.

The Core also has a BD FACSVantage used for cell sorting. This instrument is equipped with 3 lasers (488, 325 and 633nm). It provides 5-color analytic capability and two-way sorting.

All services are available to investigators within Cleveland Clinic and to outside institutions.

Appointments

Investigators must schedule appointments in advance for ALL flow cytometry. Currently 1-2 days notice is required; however, more notice is necessary for sorting. Appointments can be made by phone, e-mail or in person. All experiments must be accompanied by appropriate controls.

Forms

Download and print the Flow Request Form.