Research Core Services
The Hybridoma Core provides a resource for both development and production of monoclonal antibodies, including consultation regarding all aspects of monoclonal antibody production such as epitope design and screening assays. Services are performed under strict QC guidelines appropriate for antibodies that may have commercial potential. The core uses both stationary (T flasks) and suspension (roller bottle/spinner flasks) cultures for the production of monoclonal antibodies from hybridoma cell lines. For large-scale production, hybridoma cells can also be grown in Integra flasks, which typically achieve an antibody concentration of 1mg/ml with very good antibody purity. In the production of custom monoclonal antibodies, the core routinely performs the 1st and 2nd round screens for specific antibodies using ELISA or western blot analysis.
Affinity purification of antibody from tissue culture supe is done frequently with purities of > 95% achieved. The HCF also offers polyclonal antibody production on a limited basis. The polyclonal antibodies are produced in rabbits, so capacity is determined by the availability of housing in the BRU (Animal Facility).