Media Preparation Core

Services

Bacteriological Media

Media used for the growth of bacteria

Buffers

A buffer is an aqueous solution that has a highly stable pH (i.e. Phosphate and Tris Buffered Saline).

Cell Culture Media

A growth medium to support the growth of cells (i.e. RPMI and DMEM).

Endotoxin Testing

The Endoscan V software system uses Kinetic Turbidimetrics to provide quantitative Endotoxin results for in-process and end product samples. The assay sensitivity available for use is 0.06EU/mL using a standard curve of 5-0.05 EU/mL. The second method uses Endosafe-PTS, a rapid, point-of-use test system that utilizes Limulus Amebocyte Lysate (LAL) reagents in a test cartridge with a handheld spectrophotometer. The PTS can effectively be used to obtain fast, quantitative LAL test results in about 15 minutes.

For any specific Endotoxin questions please contact Charles River @ 1-800-762-7016/www.criver.com.

FBS- Heat Inactivated or Regular

Fetal Bovine Serum is the most widely used serum supplement due to its great versatility in cell culture application. It is favored for its increased levels of growth factors and its low levels of antibodies. Available in standard or heat-inactivated forms.

Insect Media

Media for insect cell culture.

LB Agar Plates

Luria Broth agar plates with antibiotics are typically used as a growth substrate with a selective growth compound for the controlled culture of bacteria. LB agar plates with ampicillin and kanamycin are available for purchase. Custom plates are also available. Lab must provide preferred additional growth compounds.

LB Broth

Luria Broth, a nutritionally rich medium used for the growth of bacteria.

Specialty and Custom Media

A custom recipe prepared according to researchers instructions or guidelines.

Sterility Testing

Verifying the sterility of our products or customer samples through QC broths, endotoxin and Mycoplasma testing.