Maciej  Zborowski,  Ph.D.

Maciej Zborowski, Ph.D.

Staff

Lerner Research Institute, 9500 Euclid Avenue, Cleveland, Ohio 44195
Phone: (216) 445-9330

 

Our laboratory investigates novel methods of cell separation for medical applications including rapid screening for cancer cells in blood, and isolation of blood-forming stem cells. Magnetic flow sorting is studied in model cell systems of human peripheral lymphocytes, cultured cell lines, bone marrow, and peripheral blood primed for apheresis, in collaboration with the Cleveland Clinic Taussig Cancer Center.

The cell-sorting performance depends on the specificity of magnetic nanoparticles used for cell tagging. The mechanics of cell sorting in a flow, in the presence of a magnetic field, is poorly understood. We study cell motion using a unique system, Cell Tracking Velocimetry (CTV), developed in collaboration with The Ohio State University. The characteristic cell velocities are correlated with the biophysical cell properties, such as cell size and cell surface marker expression used for binding of the magnetic nanoparticles to the wanted cells.

An interesting offshoot of the microscopic cell motion analysis in the magnetic field is the observation of natural cell mobility due to the presence of paramagnetic species inside the cell. In collaboration with the Center for Global Health and Diseases at Case Western Reserve University, we have demonstrated increased mobility of the malaria parasite-infected red blood cells, and its dependence on the parasite development stage. The method has been tested in the field in the malaria endemic region of Papua-New Guinea.

Lay Summary

We have developed instrumentation to measure magnetic properties of a single, live cell by measuring its motion in physiologic electrolyte solutions in strong magnetic fields and gradients. A highly automated and rapid process of cell image track acquisition and computer-aided tracking velocimetry allows us to sample a large number of cells (up to tens of thousands in under an hour) and determine cell magnetophoretic mobility distribution in a cell population. The cell magnetophoretic mobility is directly proportional to its magnetic susceptibility (relative to that of the fluid medium) or its spin density.

We have shown that the results of such quantitative magnetophoretic analysis agrees to within the experimental error with those calculated based on the low-spin to high-spin intracellular iron compound conversion in red blood cells (RBCs) and malaria parasite-infected RBCs. Other applications include intracellular iron uptake by mammalian cells and paramagnetic metal incorporation by bacteria.

Such information is inaccessible by the current, state-of-the-art magnetic susceptometers limited to measuring the bulk magnetic properties of matter. Thus, the cell magnetophoretic mobility analysis offers unique opportunities to probe the magnetic properties at a single cell level and to develop more efficient magnetic cell separation devices for use in cancer research and infectious diseases research.


View publications for Maciej Zborowski, Ph.D.

Weigand MRH, Gómez-Pastora J, Kim J, Kurek MT, Hickey RJ, Irwin DC, Buehler PW, Zborowski M, Palmer AF, Chalmers JJ. Magnetophoretic and spectral characterization of oxyhemoglobin and deoxyhemoglobin: Chemical versus enzymatic processes. PLoS One. 2021 Sep 3;16(9):e0257061. doi: 10.1371/journal.pone.0257061. PMID: 34478473; PMCID: PMC8415601.

Jenifer Gómez-Pastora, James Kim, Victor Multanen, Mitchell Weigand, Nicole A Walters, Eduardo Reátegui, Andre F Palmer, Mark H Yazer, Maciej Zborowski, Jeffrey J Chalmers. Intrinsically magnetic susceptibility in human blood and its potential impact on cell separation: Non-classical and intermediate monocytes have the strongest magnetic behavior in fresh human blood. Exp Hematol. 2021 May 17;S0301-472X(21)00194-6. doi: 10.1016/j.exphem.2021.05.003. Online ahead of print.

Androjna C, Yee CS, White CR, Waldorff EI, Ryaby JT, Zborowski M, Alliston T, Midura RJ. A comparison of alendronate to varying magnitude PEMF in mitigating bone loss and altering bone remodeling in skeletally mature osteoporotic rats. Bone. 2021 Feb;143:115761. doi: 10.1016/j.bone.2020.115761.

Williams PS, Moore LR, Joshi P, Goodin M, Zborowski M, Fleischman A. Microfluidic chip for graduated magnetic separation of circulating tumor cells by their epithelial cell adhesion molecule expression and magnetic nanoparticle binding. J Chromatogr A. 2021 Jan 25;1637:461823. doi: 10.1016/j.chroma.2020.461823. Epub 2020 Dec 17. PMID: 33385746; PMCID: PMC7827554.

Williams PS, Moore LR, Joshi P, Goodin M, Zborowski M, Fleischman A. J Chromatogr A. 2021 Jan 25;1637:461823. doi: 10.1016/j.chroma.2020.461823. Epub 2020 Dec 17. PMID: 33385746 

Gómez-Pastora J, Wu X, Sundar N, Alawi J, Nabar G, Winter JO, Zborowski M, Chalmers JJ. Self-Assembly and sedimentation of 5 nm SPIONs using horizontal, high magnetic fields and gradients. Sep Purif Technol. 2020 Oct;248:117012. doi: 10.1016/j.seppur.2020.117012. Epub 2020 May 4. PMID: 32655283 

Gómez-Pastora J, Weigand M, Kim J, Wu X, Strayer J, Palmer AF, Zborowski M, Yazer M, Chalmers JJ. Hyperferritinemia in critically ill COVID-19 patients - Is ferritin the product of inflammation or a pathogenic mediator? Clin Chim Acta. 2020 Oct;509:249-251. doi: 10.1016/j.cca.2020.06.033. Epub 2020 Jun 21. PMID: 32579952 . Review. 

Kim J, Gómez-Pastora J, Gilbert CJ, Weigand M, Walters NA, Reátegui E, Palmer AF, Yazer M, Zborowski M, Chalmers JJ. Quantification of the Mean and Distribution of Hemoglobin Content in Normal Human Blood Using Cell Tracking Velocimetry. Anal Chem. 2020 Jan 21;92(2):1956-1962. doi: 10.1021/acs.analchem.9b04302. Epub 2020 Jan 7. PMID: 31874030 

Kim J, Gómez-Pastora J, Weigand M, Potgieter M, A Walters N, Reátegui E, F Palmer A, Yazer M, Zborowski M, Chalmers JJ. A Subpopulation of Monocytes in Normal Human Blood Has Significant Magnetic Susceptibility: Quantification and Potential Implications. Cytometry A. 2019 May;95(5):478-487. doi: 10.1002/cyto.a.23755. Epub 2019 Apr 8. PMID: 30958642 .

Park KJ, Kim J, Testoff T, Adams J, Poklar M, Zborowski M, Venere M, Chalmers JJ. Quantitative characterization of the regulation of iron metabolism in glioblastoma stem-like cells using magnetophoresis. Biotechnol Bioeng. 2019 Jul;116(7):1644-1655. doi: 10.1002/bit.26973. Epub 2019 Apr 24. PMID: 30906984 .

Xue W, Moore LR, Nakano N, Chalmers JJ, Zborowski M. Single cell magnetometry by magnetophoresis vs. bulk cell suspension magnetometry by SQUID-MPMS - a comparison. J Magn Magn Mater. 2019 Mar 15;474:152-160. doi: 10.1016/j.jmmm.2018.10.108. Epub 2018 Oct 28. PMID: 32863537 .

Kim J , Weigand M , Palmer AF , Zborowski M , Yazer MH , Chalmers JJ . Single cell analysis of aged RBCs: quantitative analysis of the aged cells and byproducts. Analyst. 2019 Jan 28;144(3):935-942. doi: 10.1039/c8an01904e. PMID: 30617361 .

Moore LR, Mizutani D, Tanaka T, Buck A, Yazer M, Zborowski M, Chalmers JJ. Continuous, intrinsic magnetic depletion of erythrocytes from whole blood with a quadrupole magnet and annular flow channel; pilot scale study. Biotechnol Bioeng. 2018 Jun;115(6):1521-1530. doi: 10.1002/bit.26581. Epub 2018 Mar 13. PMID: 29476625 .

Sun J, Moore L, Xue W, Kim J, Zborowski M, Chalmers JJ. Correlation of simulation/finite element analysis to the separation of intrinsically magnetic spores and red blood cells using a microfluidic magnetic deposition system. Biotechnol Bioeng. 2018 May;115(5):1288-1300. doi: 10.1002/bit.26550. Epub 2018 Feb 9. PMID: 29337367 .

Moore LR, Williams PS, Chalmers JJ, Zborowski M. Tessellated permanent magnet circuits for flow-through, open gradient separations of weakly magnetic materials. J Magn Magn Mater. 2017 Apr 1;427:325-330. doi: 10.1016/j.jmmm.2016.11.027. Epub 2016 Nov 15. PMID: 29104346 .

Sivaraman B, Swaminathan G, Moore L, Fox J, Seshadri D, Dahal S, Stoilov I, Zborowski M, Mecham R, Ramamurthi A. Magnetically-responsive, multifunctional drug delivery nanoparticles for elastic matrix regenerative repair. Acta Biomater. 2017 Apr 1;52:171-186. doi: 10.1016/j.actbio.2016.11.048. Epub 2016 Nov 21. PMID: 27884774

Multiparameter Evaluation of the Heterogeneity of Circulating Tumor Cells Using Integrated RNA In Situ Hybridization and Immunocytochemical Analysis. Wu Y, Park KJ, Deighan C, Amaya P, Miller B, Pan Q, Zborowski M, Lustberg M, Chalmers J. Front Oncol. 2016 Nov 8;6:234. doi: 10.3389/fonc.2016.00234. eCollection 2016. PMID: 27878106 .

Joshi P, Kooshki M, Aldrich W, Varghai D, Zborowski M, Singh AD, Triozzi PL. Expression of natural killer cell regulatory microRNA by uveal melanoma cancer stem cells. Clin Exp Metastasis. 2016 Dec;33(8):829-838. doi: 10.1007/s10585-016-9815-9. Epub 2016 Aug 26. PMID: 27565163

Sumari D, Grimberg BT, Blankenship D, Mugasa J, Mugittu K, Moore L, Gwakisa P, Zborowski M. Application of magnetic cytosmear for the estimation of Plasmodium falciparum gametocyte density and detection of asexual stages in asymptomatic children. Malar J. 2016 Feb 24;15:113. doi: 10.1186/s12936-016-1170-4. PMID: 26911917 .

Swaminathan G, Sivaraman B, Moore L, Zborowski M, Ramamurthi A. Magnetically Responsive Bone Marrow Mesenchymal Stem Cell-Derived Smooth Muscle Cells Maintain Their Benefits to Augmenting Elastic Matrix Neoassembly. Tissue Eng Part C Methods. 2016 Apr;22(4):301-11. doi: 10.1089/ten.TEC.2015.0349. Epub 2016 Mar 18. PMID: 26830683 .

Joshi P, Williams PS, Moore LR, Caralla T, Boehm C, Muschler G, Zborowski M. Circular Halbach array for fast magnetic separation of hyaluronan-expressing tissue progenitors. Anal Chem. 2015 Oct 6;87(19):9908-15. doi: 10.1021/acs.analchem.5b02431. PMID: 26368657 .

Buck A, Moore LR, Lane CD, Kumar A, Stroff C, White N, Xue W, Chalmers JJ, Zborowski M.Magnetic separation of algae genetically modified for increased intracellular iron uptake. J Magn Magn Mater. 2015 Apr 15;380:201-204. doi: 10.1016/j.jmmm.2014.09.008. Epub 2014 Sep 10. PMID: 29353957 .

Joshi P, Jacobs B, Derakhshan A, Moore LR, Elson P, Triozzi PL, Borden E, Zborowski M. Enrichment of circulating melanoma cells (CMCs) using negative selection from patients with metastatic melanoma. Oncotarget. 2014 May 15;5(9):2450-61. doi: 10.18632/oncotarget.1683. PMID: 24811334 .

Androjna C, Fort B, Zborowski M, Midura RJ. Pulsed electromagnetic field treatment enhances healing callus biomechanical properties in an animal model of osteoporotic fracture. Bioelectromagnetics. 2014 Sep;35(6):396-405. doi: 10.1002/bem.21855. Epub 2014 Apr 24. PMID: 24764277

Moore LR, Williams PS, Nehl F, Abe K, Chalmers JJ, Zborowski M. Feasibility study of red blood cell debulking by magnetic field-flow fractionation with step-programmed flow. Anal Bioanal Chem. 2014 Feb;406(6):1661-70. doi: 10.1007/s00216-013-7394-z. Epub 2013 Oct 19. PMID: 24141316 .

Wu Y, Deighan CJ, Miller BL, Balasubramanian P, Lustberg MB, Zborowski M, Chalmers JJ. Isolation and analysis of rare cells in the blood of cancer patients using a negative depletion methodology. Methods. 2013 Dec 1;64(2):169-82. doi: 10.1016/j.ymeth.2013.09.006. Epub 2013 Sep 20. PMID: 24056212 .

Joshi P, Zborowski M, Triozzi PL. Circulating melanoma cells: scoping the target. Front Oncol. 2013 Aug 12;3:189. doi: 10.3389/fonc.2013.00189. eCollection 2013. PMID: 23964346 .

Orita T, Moore LR, Joshi P, Tomita M, Horiuchi T, Zborowski M. A quantitative determination of magnetic nanoparticle separation using on-off field operation of quadrupole magnetic field-flow fractionation (QMgFFF). Anal Sci. 2013;29(7):761-4. doi: 10.2116/analsci.29.761. PMID: 23842422 .

Moore LR, Nehl F, Dorn J, Chalmers JJ, Zborowski M. Open Gradient Magnetic Red Blood Cell Sorter Evaluation on Model Cell Mixtures. IEEE Trans Magn. 2013 Feb;49(1):309-315. doi: 10.1109/tmag.2012.2225098. PMID: 24910468 .

Caralla T, Joshi P, Fleury S, Luangphakdy V, Shinohara K, Pan H, Boehm C, Vasanji A, Hefferan TE, Walker E, Yaszemski M, Hascall V, Zborowski M, Muschler GF. In vivo transplantation of autogenous marrow-derived cells following rapid intraoperative magnetic separation based on hyaluronan to augment bone regeneration. Tissue Eng Part A. 2013 Jan;19(1-2):125-34. doi: 10.1089/ten.tea.2011.0622. Epub 2012 Oct 19. PMID: 23082937 .

Xu J, Mahajan K, Xue W, Winter JO, Zborowski M, Chalmers JJ. Simultaneous, single particle, magnetization and size measurements of micron sized, magnetic particles. J Magn Magn Mater. 2012 Dec 1;324(24):4189-4199. doi: 10.1016/j.jmmm.2012.07.039. PMID: 22962515 .

Jin X, Abbot S, Zhang X, Kang L, Voskinarian-Berse V, Zhao R, Kameneva MV, Moore LR, Chalmers JJ, Zborowski M. Erythrocyte enrichment in hematopoietic progenitor cell cultures based on magnetic susceptibility of the hemoglobin. PLoS One. 2012;7(8):e39491. doi: 10.1371/journal.pone.0039491. Epub 2012 Aug 27. PMID: 22952572 .

Balasubramanian P, Lang JC, Jatana KR, Miller B, Ozer E, Old M, Schuller DE, Agrawal A, Teknos TN, Summers TA Jr, Lustberg MB, Zborowski M, Chalmers JJ. Multiparameter analysis, including EMT markers, on negatively enriched blood samples from patients with squamous cell carcinoma of the head and neck. PLoS One. 2012;7(7):e42048. doi: 10.1371/journal.pone.0042048. Epub 2012 Jul 26. PMID: 22844540 .

Lustberg M, Jatana KR, Zborowski M, Chalmers JJ. Emerging technologies for CTC detection based on depletion of normal cells. Recent Results Cancer Res. 2012;195:97-110. doi: 10.1007/978-3-642-28160-0_9. PMID: 22527498 . Review.

Jin X, Chalmers JJ, Zborowski M. Iron transport in cancer cell culture suspensions measured by cell magnetophoresis. Anal Chem. 2012 May 15;84(10):4520-6. doi: 10.1021/ac3004677. Epub 2012 Apr 26. PMID: 22500468 .

Zborowski M, Chalmers JJ. Rare cell separation and analysis by magnetic sorting. Anal Chem. 2011 Nov 1;83(21):8050-6. doi: 10.1021/ac200550d. Epub 2011 Aug 19. PMID: 21812408 .

Jin X, Yazer MH, Chalmers JJ, Zborowski M. Quantification of changes in oxygen release from red blood cells as a function of age based on magnetic susceptibility measurements. Analyst. 2011 Jul 21;136(14):2996-3003. doi: 10.1039/c0an01018a. Epub 2011 Jun 6. PMID: 21647486 .

Sun J, Zborowski M, Chalmers JJ. Quantification of both the presence, and oxidation state, of Mn in Bacillus atrophaeus spores and its imparting of magnetic susceptibility to the spores. Biotechnol Bioeng. 2011 May;108(5):1119-29. doi: 10.1002/bit.23034. Epub 2011 Jan 4. PMID: 21449026 .

Hoyos M, Moore L, Williams PS, Zborowski M. The use of a linear Halbach array combined with a step-SPLITT channel for continuous sorting of magnetic species. J Magn Magn Mater. 2011 May 1;323(10):1384-1388. doi: 10.1016/j.jmmm.2010.11.051. PMID: 21399709 .

Williams PS, Carpino F, Zborowski M. Characterization of magnetic nanoparticles using programmed quadrupole magnetic field-flow fractionation. Philos Trans A Math Phys Eng Sci. 2010 Sep 28;368(1927):4419-37. doi: 10.1098/rsta.2010.0133. PMID: 20732895 .

Williams PS, Carpino F, Zborowski M. Theory for nanoparticle retention time in the helical channel of quadrupole magnetic field-flow fractionation. J Magn Magn Mater. 2009 May 1;321(10):1446-1451. doi: 10.1016/j.jmmm.2009.02.065. PMID: 20161002 .

Zborowski M, Moore LR, Williams PS, Chalmers JJ Magnetic Pressure as a Scalar Representation of Field Effects in Magnetic Suspensions.. AIP Conf Proc. 2010;1311:111-117. doi: 10.1063/1.3529999. PMID: 25382882 .

Schneider T, Karl S, Moore LR, Chalmers JJ, Williams PS, Zborowski M. Sequential CD34 cell fractionation by magnetophoresis in a magnetic dipole flow sorter. Analyst. 2010 Jan;135(1):62-70. doi: 10.1039/b908210g. Epub 2009 Nov 4. PMID: 20024182 .

Labhasetwar V, Zborowski M, Abramson AR, Basilion JP. Nanoparticles for imaging, diagnosis, and therapeutics. Mol Pharm. 2009 Sep-Oct;6(5):1261-2. doi: 10.1021/mp9001798. PMID: 19799461

Williams PS, Carpino F, Zborowski M. Magnetic nanoparticle drug carriers and their study by quadrupole magnetic field-flow fractionation. Mol Pharm. 2009 Sep-Oct;6(5):1290-306. doi: 10.1021/mp900018v. PMID: 19591456 . Review.

Balasubramanian P, Yang L, Lang JC, Jatana KR, Schuller D, Agrawal A, Zborowski M, Chalmers JJ. Confocal images of circulating tumor cells obtained using a methodology and technology that removes normal cells. Mol Pharm. 2009 Sep-Oct;6(5):1402-8. doi: 10.1021/mp9000519. PMID: 19445481 .

Jin X, Zhao Y, Richardson A, Moore L, Williams PS, Zborowski M, Chalmers JJ. Differences in magnetically induced motion of diamagnetic, paramagnetic, and superparamagnetic microparticles detected by cell tracking velocimetry. Analyst. 2008 Dec;133(12):1767-75. doi: 10.1039/b802113a. Epub 2008 Sep 9. PMID: 19082082 .

Nath P, Strelnik J, Vasanji A, Moore LR, Williams PS, Zborowski M, Roy S, Fleischman AJ. Development of multistage magnetic deposition microscopy. Anal Chem. 2009 Jan 1;81(1):43-9. doi: 10.1021/ac8010186. PMID: 19055419

Yang L, Lang JC, Balasubramanian P, Jatana KR, Schuller D, Agrawal A, Zborowski M, Chalmers JJ. Optimization of an enrichment process for circulating tumor cells from the blood of head and neck cancer patients through depletion of normal cells. Biotechnol Bioeng. 2009 Feb 1;102(2):521-34. doi: 10.1002/bit.22066. PMID: 18726961 .

Jing Y, Mal N, Williams PS, Mayorga M, Penn MS, Chalmers JJ, Zborowski M. Quantitative intracellular magnetic nanoparticle uptake measured by live cell magnetophoresis. FASEB J. 2008 Dec;22(12):4239-47. doi: 10.1096/fj.07-105544. Epub 2008 Aug 25. PMID: 18725459 .

Williams PS, Hoyos M, Kurowski P, Salhi D, Moore LR, Zborowski M. Characterization of nonspecific crossover in split-flow thin channel fractionation. Anal Chem. 2008 Sep 15;80(18):7105-15. doi: 10.1021/ac800841q. Epub 2008 Aug 13. PMID: 18698797 .

Karl S, David M, Moore L, Grimberg BT, Michon P, Mueller I, Zborowski M, Zimmerman PA. Enhanced detection of gametocytes by magnetic deposition microscopy predicts higher potential for Plasmodium falciparum transmission. Malar J. 2008 Apr 25;7:66. doi: 10.1186/1475-2875-7-66. PMID: 18439240 .

Said TM, Agarwal A, Zborowski M, Grunewald S, Glander HJ, Paasch U. Utility of magnetic cell separation as a molecular sperm preparation technique. J Androl. 2008 Mar-Apr;29(2):134-42. doi: 10.2164/jandrol.107.003632. Epub 2007 Dec 12. PMID: 18077822 . Review.

Tong X, Xiong Y, Zborowski M, Farag SS, Chalmers JJ. A novel high throughput immunomagnetic cell sorting system for potential clinical scale depletion of T cells for allogeneic stem cell transplantation. Exp Hematol. 2007 Oct;35(10):1613-22. doi: 10.1016/j.exphem.2007.06.015. Epub 2007 Aug 13. PMID: 17697744 .

Contado C, Dalpiaz A, Leo E, Zborowski M, Williams PS. Complementary use of flow and sedimentation field-flow fractionation techniques for size characterizing biodegradable poly(lactic acid) nanospheres. J Chromatogr A. 2007 Jul 20;1157(1-2):321-35. doi: 10.1016/j.chroma.2007.04.038. Epub 2007 Apr 21. PMID: 17482199 .

Jing Y, Moore LR, Schneider T, Williams PS, Chalmers JJ, Farag SS, Bolwell B, Zborowski M. Negative selection of hematopoietic progenitor cells by continuous magnetophoresis. Exp Hematol. 2007 Apr;35(4):662-72. doi: 10.1016/j.exphem.2006.12.009. PMID: 17379076

Melnik K, Sun J, Fleischman A, Roy S, Zborowski M, Chalmers JJ. Quantification of magnetic susceptibility in several strains of Bacillus spores: implications for separation and detection. Biotechnol Bioeng. 2007 Sep 1;98(1):186-92. doi: 10.1002/bit.21400. PMID: 17335063

Tong X, Yang L, Lang JC, Zborowski M, Chalmers JJ. Application of immunomagnetic cell enrichment in combination with RT-PCR for the detection of rare circulating head and neck tumor cells in human peripheral blood. Cytometry B Clin Cytom. 2007 Sep;72(5):310-23. doi: 10.1002/cyto.b.20177. PMID: 17205568

Jing Y, Moore LR, Williams PS, Chalmers JJ, Farag SS, Bolwell B, Zborowski M. Blood progenitor cell separation from clinical leukapheresis product by magnetic nanoparticle binding and magnetophoresis. Biotechnol Bioeng. 2007 Apr 15;96(6):1139-54. doi: 10.1002/bit.21202. PMID: 17009321

Zhang H, Williams PS, Zborowski M, Chalmers JJ. Binding affinities/avidities of antibody-antigen interactions: quantification and scale-up implications. Biotechnol Bioeng. 2006 Dec 5;95(5):812-29. doi: 10.1002/bit.21024. PMID: 16937410

Zborowski M, Kligman B, Midura RJ, Wolfman A, Patterson TE, Ibiwoye M, Grabiner M. Decibel attenuation of pulsed electromagnetic field (PEMF) in blood and cortical bone determined experimentally and from the theory of ohmic losses. Ann Biomed Eng. 2006 Jun;34(6):1030-41. doi: 10.1007/s10439-006-9126-4. Epub 2006 May 18. PMID: 16783658

Patterson TE, Sakai Y, Grabiner MD, Ibiwoye M, Midura RJ, Zborowski M, Wolfman A. Exposure of murine cells to pulsed electromagnetic fields rapidly activates the mTOR signaling pathway. Bioelectromagnetics. 2006 Oct;27(7):535-44. doi: 10.1002/bem.20244. PMID: 16715521

Schneider T, Moore LR, Jing Y, Haam S, Williams PS, Fleischman AJ, Roy S, Chalmers JJ, Zborowski M. Continuous flow magnetic cell fractionation based on antigen expression level. J Biochem Biophys Methods. 2006 Jul 31;68(1):1-21. doi: 10.1016/j.jbbm.2006.02.011. Epub 2006 Mar 29. PMID: 16675023

Zimmerman PA, Thomson JM, Fujioka H, Collins WE, Zborowski M. Diagnosis of malaria by magnetic deposition microscopy. Am J Trop Med Hyg. 2006 Apr;74(4):568-72. PMID: 16606985 .

Lara O, Tong X, Zborowski M, Farag SS, Chalmers JJ. Comparison of two immunomagnetic separation technologies to deplete T cells from human blood samples. Biotechnol Bioeng. 2006 May 5;94(1):66-80. doi: 10.1002/bit.20807. PMID: 16518837

Moore LR, Fujioka H, Williams PS, Chalmers JJ, Grimberg B, Zimmerman PA, Zborowski M. Hemoglobin degradation in malaria-infected erythrocytes determined from live cell magnetophoresis. FASEB J. 2006 Apr;20(6):747-9. doi: 10.1096/fj.05-5122fje. Epub 2006 Feb 6. PMID: 16461330 .

Sakai Y, Patterson TE, Ibiwoye MO, Midura RJ, Zborowski M, Grabiner MD, Wolfman A. Exposure of mouse preosteoblasts to pulsed electromagnetic fields reduces the amount of mature, type I collagen in the extracellular matrix. J Orthop Res. 2006 Feb;24(2):242-53. doi: 10.1002/jor.20012. PMID: 16435357 .

Benincasa MA, Moore LR, Williams PS, Poptic E, Carpino F, Zborowski M. Cell sorting by one gravity SPLITT fractionation. Anal Chem. 2005 Aug 15;77(16):5294-301. doi: 10.1021/ac058013o. PMID: 16097771

Powell KA, Latson L, Ibiwoye MO, Wolfman A, Grabiner MD, Zborowski M, Sakai Y, Midura RJ. In vivo longitudinal assessment of bone resorption in a fibular osteotomy model using micro-computed tomography. Iowa Orthop J. 2005;25:123-8. PMID: 16089084 .

Leigh DR, Steinert S, Moore LR, Chalmers JJ, Zborowski M. Cell tracking velocimetry as a tool for defining saturation binding of magnetically conjugated antibodies. Cytometry A. 2005 Aug;66(2):103-8. doi: 10.1002/cyto.a.20155. PMID: 15973696 .

Midura RJ, Ibiwoye MO, Powell KA, Sakai Y, Doehring T, Grabiner MD, Patterson TE, Zborowski M, Wolfman A. Pulsed electromagnetic field treatments enhance the healing of fibular osteotomies. J Orthop Res. 2005 Sep;23(5):1035-46. doi: 10.1016/j.orthres.2005.03.015. PMID: 15936919 .

Zhang H, Moore LR, Zborowski M, Williams PS, Margel S, Chalmers JJ. Establishment and implications of a characterization method for magnetic nanoparticle using cell tracking velocimetry and magnetic susceptibility modified solutions. Analyst. 2005 Apr;130(4):514-27. doi: 10.1039/b412723d. Epub 2005 Feb 17. PMID: 15776162

Zborowski M, Chalmers JJ. Magnetic cell sorting. Methods Mol Biol. 2005;295:291-300. doi: 10.1385/1-59259-873-0:291. PMID: 15596904

Lara O, Tong X, Zborowski M, Chalmers JJ. Enrichment of rare cancer cells through depletion of normal cells using density and flow-through, immunomagnetic cell separation. Exp Hematol. 2004 Oct;32(10):891-904. doi: 10.1016/j.exphem.2004.07.007. PMID: 15504544

Ibiwoye MO, Powell KA, Grabiner MD, Patterson TE, Sakai Y, Zborowski M, Wolfman A, Midura RJ. Bone mass is preserved in a critical-sized osteotomy by low energy pulsed electromagnetic fields as quantitated by in vivo micro-computed tomography. J Orthop Res. 2004 Sep;22(5):1086-93. doi: 10.1016/j.orthres.2003.12.017. PMID: 15304283 .

Moore LR, Milliron S, Williams PS, Chalmers JJ, Margel S, Zborowski M. Control of magnetophoretic mobility by susceptibility-modified solutions as evaluated by cell tracking velocimetry and continuous magnetic sorting. Anal Chem. 2004 Jul 15;76(14):3899-907. doi: 10.1021/ac049910f. PMID: 15253623

McCloskey KE, Chalmers JJ, Zborowski M. Magnetic cell separation: characterization of magnetophoretic mobility. Anal Chem. 2003 Dec 15;75(24):6868-74. doi: 10.1021/ac034315j. PMID: 14670047

Williams PS, Decker K, Nakamura M, Chalmers JJ, Moore LR, Zborowski M. Splitter imperfections in annular split-flow thin separation channels: experimental study of nonspecific crossover. Anal Chem. 2003 Dec 1;75(23):6687-95. doi: 10.1021/ac030152n. PMID: 14640746

McCloskey KE, Moore LR, Hoyos M, Rodriguez A, Chalmers JJ, Zborowski M. Magnetophoretic cell sorting is a function of antibody binding capacity. Biotechnol Prog. 2003 May-Jun;19(3):899-907. doi: 10.1021/bp020285e. PMID: 12790655

Zborowski M, Ostera GR, Moore LR, Milliron S, Chalmers JJ, Schechter AN. Red blood cell magnetophoresis. Biophys J. 2003 Apr;84(4):2638-45. doi: 10.1016/S0006-3495(03)75069-3. PMID: 12668472 .

Williams PS, Moore LR, Chalmers JJ, Zborowski M. Splitter imperfections in annular split-flow thin separation channels: effect on nonspecific crossover. Anal Chem. 2003 Mar 15;75(6):1365-73. doi: 10.1021/ac020649h. PMID: 12659197

Zborowski M, Midura RJ, Wolfman A, Patterson T, Ibiwoye M, Sakai Y, Grabiner M. Magnetic field visualization in applications to pulsed electromagnetic field stimulation of tissues. Ann Biomed Eng. 2003 Feb;31(2):195-206. doi: 10.1114/1.1540104. PMID: 12627827

Chosy EJ, Nakamura M, Melnik K, Comella K, Lasky LC, Zborowski M, Chalmers JJ. Characterization of antibody binding to three cancer-related antigens using flow cytometry and cell tracking velocimetry. Biotechnol Bioeng. 2003 May 5;82(3):340-51. doi: 10.1002/bit.10581.


US Patent Patent Title Issue Date First-Named Inventor
6,557,430 System And Device For Determining Particle Characteristics 5/6/2003 Maciej Zborowski Ph.D
6,467,630 Continuous Particle And Molecule Separation With An Annular Flow Channel 10/22/2002 Maciej Zborowski Ph.D
6,412,359 System And Device For Determining Particle Characteristics 7/2/2002 Maciej Zborowski Ph.D
6,142,025 Method For Determining Particale Characteristics 11/7/2000 Maciej Zborowski Ph.D
6,120,735 Fractional cell sorter 9/19/2000 Maciej Zborowski Ph.D
6,082,205 System And Device For Determining Particle Characteristics 7/4/2000 Maciej Zborowski Ph.D
5,974,901 Method For Determining Particle Characteristics 11/2/1999 Maciej Zborowski Ph.D
5,968,820 Method For Magnetically Separating Cells Into Fractionated Flow Streams 10/19/1999 Maciej Zborowski Ph.D

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