Genomics Core

About

The mission of Lerner Research Institute's Genomics Core is to provide easy access to state-of-the-art DNA and RNA services to Cleveland Clinic investigators, as well as external academic and commercial users.

The Genomics Core uses cutting-edge genomics technology to generate top-quality data with a rapid turn-around time at an affordable cost. We offer a wide range of services, including Illumina microarray-based gene expression profiling, genotyping and next-generation sequencing (NGS). Our lab is equipped with the renowned Illumina MiSeq desktop sequencer and the Illumina NovaSeq 6000, which enable rapid and effective targeted and whole genome analyses. We prepare and normalize libraries for RNA-seq, miRNA-seq, DNA-Seq, targeted exome sequencing, amplicon sequencing, bacterial 16S surveys, whole genome sequencing and single-cell RNA-seq. Other services include DNA/RNA QC on an Agilent Technologies Bioanalyzer and Tapestation systems, shearing of DNA and chromatin with Covaris S2 technology and microarray data analysis support. We also collaborate with the Genomic Medicine Biorepository to extract DNA and RNA for our users.

The Genomics Core team can work with individual users to provide services not listed here. We also provide letters of support for federal and nonfederal grant applications and are open to research collaborations. Please contact the Genomics Core at LRIgenomics@ccf.org or 216.444.7124 for service requests and daily communication. All scientific questions or questions on design should be directed to Dr. Cheng at chengy@ccf.org. All requests for letters of support for grant applications should be directed to Dr. Eng at engc@ccf.org. All other questions can be directed to LRIgenomics@ccf.org.


Contacts

Charis  Eng, MD, PhD

Charis Eng, MD, PhD
Department Chair
Sondra J. and Stephen R. Hardis Endowed Chair in Cancer Genomic Medicine
Location:NE5-314
Phone:(216) 444-3440
engc@ccf.org
Research Profile

Yu-Wei  Cheng, PhD

Yu-Wei Cheng, PhD
Staff
Scientific Manager of Genomics Core
Location:LL2-139
Phone:(216) 445-0757
chengy@ccf.org

Dean  Horton, PhD

Dean Horton, PhD
Program Manager III
Genomics Core
Location:R4-058
Phone:(216) 444-7124
hortond5@ccf.org

Min Hui  Lim

Min Hui Lim
Senior Research Technologist
Phone:(216) 444-7124
limm3@ccf.org

Evan  Welch

Evan Welch
Research Technician
Location:R4-058
Phone:(216) 444-7124
welche@ccf.org

Services







Equipment








Grant Information

The Genomics Core offers a wide range of genomics services, including high-throughput sequencing, single-cell RNA sequencing, DNA and RNA library preparation, microarray and genotyping applications, and sample quality control. We are equipped with a Novaseq 6000 and MiSeq systems for a variety of sequencing applications, including but not limited to RNA-seq, scRNA-seq, miRNA-seq, DNA-seq, Whole Exome Sequencing (WES), Whole Genome Sequencing (WGS), amplicon sequencing, and microbial metagenomics and metatranscriptomics. The Genomics Core utilizes an Illumina iScan for genotyping/microarray chip scanning, and sample quality control using an Agilent 2100 Bioanalyzer, 4200 Tapestation, Qubit Fluorometer, qPCR, and a Covaris S2 sonicator. In addition, the Genomics Core is a hub for all external capillary sequencing and oligo synthesis needs for Cleveland Clinic researchers. An Ingenuity Pathway Analysis (IPA) license is maintained by the genomics core and is available to users of the genomics and/or proteomics cores. The Genomics Core is a shared resource of the NCI designated Case Comprehensive Cancer Center (P30CA043703-28) and the NIH Cleveland Clinical and Translational Science Collaborative (CTSC). 

Several library preparation options which are available for researchers interested in RNA-seq or DNA-seq, including samples with degraded or low-concentration genomic material. In collaboration with the Flow Cytometry Core, the Genomics Core prepares libraries of 10X single cell RNA transcriptomes, which allows for the simultaneous processing of up to ten-thousand individual cells per sample. Constructed genomic libraries can be assessed for quality using microfluidic chips which allow for accurate quantification of RNA and DNA fragments. Genomic libraries are consecutively sequenced on one of our Illumina HTS platforms with the ability to provide between 300 Mb – 500 Gb of data, and can sequence fragments up to 300 bp in length (either unidirectional or paired-end). Genomics Core personnel are here to assist for consultation on which library prep and sequencing options are best for your experiment, resulting in the most cost-effective and robust data sets you need to confidently explore answers to your research questions.

The Genomics Core team can work with individual users to provide services not listed here. We collaborate with the Genomic Medicine Biorepository for customers who need tissues processed to nucleic acids. We also provide letters of support for federal and nonfederal grant applications and are open to research collaborations. Please contact LRI Genomics Core at LRIgenomics@ccf.org or Dr. Horton at hortond5@ccf.org or 216.444.7124 for service requests and daily communication. All scientific questions/questions on design should be directed to Dr. Cheng at chengy@ccf.org. All requests for letters of support for grant applications should be directed to Dr. Eng at engc@ccf.org.

Genomics Core

  • Genomics Lab
    R4-058
    216.444.7124
    LRIgenomics@ccf.org
    8950 Euclid Ave. R4
    Cleveland, OH 44195
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