John W. Crabb, Ph.D.
Lerner Research Institute,
9500 Euclid Avenue, Cleveland, Ohio 44195
Goals and Projects
- The long-term goal of our uveal melanoma project is the development of a multi-antibody immunological assay for UM metastasis that will complement current cytogenetic and genetic prognostic methods and establish for the first time methods to detect and quantify circulating uveal melanoma tumor cells.
- The long-term goals of our glaucoma projects are to better understand the molecular mechanisms of glaucomatous vision loss, identify therapeutic targets and develop a panel of blood-borne glaucoma biomarkers.
- The long-term goal of our age-related macular degeneration (AMD) project is the development of molecular technology for assessing AMD risk and monitoring AMD therapeutics.
Crabb, JW, M Miyagi, X Gu, K Shadrach, KA West, H Sakaguchi, M Kamei, A Hasan, L Yan, ME Rayborn, RG Salomon, JG Hollyfield (2002) Drusen Proteome Analysis: an Approach to the Etiology of Age-Related Macular Degeneration Proc Natl Acad Sci USA 99:14682-14687.
Miyagi M, H Sakaguchi, RM Darrow, L Yan, KA West, KS Aulak, DJ Stuehr, JG Hollyfield, DT Organisciak and JW Crabb (2002) Evidence that Light Modulates Protein Nitration in Rat Retina. Molecular and Cellular Proteomics 1: 293-303.
Golovleva I, S Bhattacharya, Z.Wu, N Shaw, Y Yang, K Andrabi, KA West , MS Burstedt, K Forsman, G Holmgren, O Sandgren, N Noy , J Qin and JW.Crabb (2003) Disease Causing Mutations in the Cellular Retinaldehyde-binding Protein Tighten as well as Abolish Retinoid Interactions. J Biol Chem 278: 12397-12402.
Bhattacharya SK, EJ Rockwood, SD Smith, VL Bonilha, JS Crabb, RW Kuchtey, NG Robertson, NS Peachey, CC Morton and JW Crabb(2005) Proteomics Reveals Cochlin Deposits Associated With Glaucomatous Trabecular Meshwork. J Biol Chem 280: 6080 – 6084.
EbrahemQ, K Renganathan, J Sears, A Vasanji, X Gu, L Lu, RG Salomon, JW Crabb, B Anand-Apte (2006) Carboxyethylpyrrole Oxidative Protein Modifications Stimulate Neovascularization: Implications for Age-Related Macular Degeneration Proc Nal Acad Sci USA 103: 13480-13484
Gu J, GJT Pauer, X Yue, U Narendra, GM Sturgill, J Bena, X Gu, NS Peachey, RG Salomon, SA Hagstrom, W Crabb and the Clinical Genomic and Proteomic AMD Study Group (2009) Assessing Susceptibility To Age-Related Macular Degeneration With Proteomic And Genomic Biomarkers. Mol & Cell Proteomics 8:1338-49.
Ni J, X Yuan, J Gu, X Yue, X Gu, RH Nagaraj, JW Crabb and The Clinical Genomic and Proteomic AMD Study Group (2009) Plasma Protein Pentosidine And Carboxymethyllysine, Biomarkers For Age-Related Macular Degeneration. Mol & Cell Proteomics 8: 1921-33.
Yuan X, X Gu, JS Crabb, X Yue, K Shadrach, JG Hollyfield and JW Crabb (2010) Quantitative Proteomics: Comparison of the Macular Bruch’s Membrane/Choroid Complex from Age-related Macular Degeneration and Normal Eyes. Mol & Cell Proteomics 9: 1031-1046.
Bollinger KE, Crabb JS, Yuan X, Putliwala T, Clark AF, Crabb JW (2011) Quantitative Proteomics: TGFβ2-Signaling in Trabecular Meshwork Cells. Invest Ophthal Visual Sci 52, 8287-8291.
Bollinger KE, Crabb JS, Yuan X, Putliwala T, Clark AF, Crabb JW (2012) Proteomic Similarities in Steroid Responsiveness In Normal and Glaucomatous Trabecular Meshwork Cells. Mol Vision 18: 2001-2011.
Renganathan K, J Gu, ME Rayborn, JS Crabb, RG Salomon, RJ Collier, MA Kapin, C Romano, JG Hollyfield, and JW Crabb (2013) CEP Biomarkers As Potential Tools for Monitoring Therapeutics, PLoS One 8:e76325.
Gu X, Hu Z, Ebrahem Q, Crabb JS, Mahfouz R, Radivoyevitch T, Crabb JW, Saunthararajah Y. (2014) Runx1 Regulation of Pu.1 Corepressor/Coactivator Exchange Identifies Specific Molecular Targets for Leukemia Differentiation Therapy. J Biol Chem 289: 14881-95.
Kim YW, Yakubenko VP, West XZ, Gugiu GB, Kutralanathan R, Biswas S, Gao D, Crabb JW, Salomon RG, Podrez E, Byzova T (2015) Receptor-Mediated Mechanism Controlling Tissue Levels of Bioactive Lipid Oxidation Products. Circ Res 117: 321-332.
Crabb JW, Hu B, Crabb JS, Triozzi P, Saunthararajah Y, Tubbs R, Singh AD (2015) iTRAQ Quantitative Proteomic Comparison of Metastatic and Non-Metastatic Uveal Melanoma Tumors. PLoS ONE 10: e0135543.
Tayou J, Wang Q, Jang GF, Pronin AN, Orlandi C, Martemyanov KA, Crabb JW, Slepak VZ (2016) Regulator of G-protein Signaling 7 (RGS7) can exist in a homo-oligomeric form that is regulated by Gαo and R7-binding protein. J Biol Chem 291, 9133-47
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