Flow Cytometry

About

The Florida Research & Innovation Center (FRIC) Flow Cytometry Core provides state-of-the-art instrumentation and technical support. The principal assets of the Core consist of analytical equipment, cell sorting capabilities, single-cell library preparation instrumentation and an expert ASCP-certified Specialist in Cytometry. Multi-parameter flow cytometric analysis is provided by a spectral flow cytometer. The Core also provides access to instrumentation capable of single cell partitioning and generation of sequencing-ready libraries, as well as to qPCR and characterization instrumentation. The Core provides access to data analysis software and analysis expertise.

Please remember to acknowledge the Flow Cytometry Core in any research published that includes data or expertise provided by the Core.

For more information on access, policies, and more, please visit the FRIC Flow Cytometry Core Connect Today page (Cleveland Clinic employees only).


Contacts

Michael  Gregory, MS

Michael Gregory, MS
Director, Flow Cytometry Core
Phone:(772) 345-8116
gregorm6@ccf.org

Services

Multi-parameter Flow Cytometry

Aurora Spectral Analyzer

Leveraging the power of full spectral flow cytometry, our four laser Aurora Spectral Analyzer can detect 20+ parameters on each cell. Auto-fluorescence subtraction and state-of-the-art detectors allow for a much higher resolution, especially for problematic cell types with high background or low expression markers. 

FACSAria Cell Sorters

FACSAria Cell Sorters are available for the physical separation of cell populations identified via flow cytometry. FACSAria sorters allow for the detection of up to 16 fluorescent parameters excited by four laser lines, as well as forward scattered and side scattered laser light. They allow sorting of up to four populations at a time, with deposition into a variety of tube types, or deposition of one population at a time into/onto well plates (up to 384) or slides.

Single Cell Partitioning for Sequencing Library Preparation

10x Chromium Controllers are available for the generation of single cell sequencing libraries. This powerful technology supports kits that include single cell gene expression and immune profiling, as well as ATAC and/or protein expression multiomics. After preparation of sequencing libraries, the Core can help coordinate sequencing offsite with Lerner Research Institute's Genomics Core and the FRIC Bioinformatics Core can assist with experiment design and data processing. 

Experiment Consultation

Assistance is available with experimental design, cell preparation troubleshooting, assay and application selection, preparation of data or methods for publication, and more. The Director of the Flow Cytometry Core is an ASCP-certified Specialist in Cytometry with over 16 years of experience in flow cytometry.

Instrument, Experimental Design and Data Analysis Training

Training on instrumentation, techniques and data analysis tools is currently offered as needed. Get trained on the Aurora spectral flow cytometer to utilize it self-serve 24/7. Training on data analysis software, including FlowJo, FCSExpress, FACSDiva and more, is available, though for basic introductory training, please see the vendor websites first. 

Data Analysis

  • Data analysis services are available - please complete a consultation form via iLab.
  • For self-serve data analysis, Flowjo or FCSExpress are recommended. FlowJo is currently available from Lerner Research Institute's Flow Cytometry Core (Cleveland) for a licensing fee. Instructions to access the software (PDF) are provided on their webpage

For service rates and scheduling, please visit our iLab page.

For more information on services and instrumentation, please visit the FRIC Flow Cytometry Connect Today page (Cleveland Clinic employees only).

Equipment

Cytek Aurora Spectral Analyzer
A spectral flow cytometer capable of 20+ parameter detection. Features include:

  • 4 lasers: 405, 488, 561 and 640nm
  • Full spectrum detection and spectral unmixing technology for high parameter flow cytometry
  • Violet side scatter detector for small particle analysis
  • High throughput sample loader (96 well plate)
  • Autofluorescence subtraction and powerful detectors for high resolution detection

Aurora Spectral Analyzer

FACSAria Cell Sorters
Cell sorters capable of flow cytometric analysis and target cell (or particle) purification. Features include:

  • 4 lasers: 405, 488, 532 and 640nm
  • 16 fluorescent parameter detectors
  • High speed sorting of up to 4 target populations at a time
  • Automated Cell Deposition Unit for plate sorting up to 384-well, slides, and more  

FACSAria

10x Chromium Controller

Partitions single cells for downstream library preparation. Applications include:

  • Single cell gene expression (3’ or 5’)
  • Single cell immune profiling
  • Single cell ATAC
  • Single cell multiomics  

10x Chromium Controller

Other Instruments:

  • Thermo Cytospin 4
  • Countess Cell Counters
  • Thermo Nanodrop 2000c
  • Agilent Bioanalyzer 2100
  • Roche LightCycler 480 qPCR
  • ThermoFisher ViiA7 qPCR
  • Bio-Rad C1000
  • Thermocycler Eppendorf 5430
  • ThermoFisher X1R Centrifuge
  • Fluorescent Microscope

For service rates and scheduling, please visit our iLab page.

For more detailed information, please visit the FRIC Flow Cytometry Connect Today page (Cleveland Clinic employees only).

Flow Cytometry

  • View prices and request services:
    Go to iLab

    Cleveland Clinic Employees Only:
    FRIC Flow Cytometry Core Connect Today