Hod  Dana,  Ph.D.

Hod Dana, Ph.D.

Assistant Staff

Assistant Professor, Molecular Medicine, CCLCM-CWRU

Lerner Research Institute, 9500 Euclid Avenue, Cleveland, Ohio 44195


"Progress in science depends on new techniques, new discoveries, and new ideas, probably in that order" --Sydney Brenner

In the lab, we develop new methods and platforms for enabling large-scale recording and manipulation of neurons in the living brain. We believe that this approach is essential for a better understanding of how the spatially-distributed and highly-interconnected circuits in the mammalian brain integrate sensory input and transform it into motor action, especially in the context of neurodegeneration or various neurologic conditions. This type of computation is also involved in almost any behavior or decision-making process we do in our everyday life; therefore, we want to gain a deeper understanding of its nature. We work with state-of-the-art optical tools, such as ultrafast lasers, nonlinear microscopy and high-harmonic generation, as well as with novel protein sensors and actuators for detecting and manipulating the brain activity.

The research in the lab is interdisciplinary and collaborative in its nature, and combines approaches from different fields, like engineering, physics, structural biology, and systems neuroscience. Our current projects focus on changes to the brain activity in rodent models of multiple sclerosis and brain injuries, and how we can protect the affected brain circuits.

Das A, Holden S, Borovicka J, Icardi J, Patel D, Patel R, Raber J, Dana H. (2022) Recording large-scale, cellular-resolution neuronal activity from freely-moving mice. BioRxiv https://doi.org/10.1101/2022.06.01.494442

Chornyy S, Das A, Borovicka JA, Patel D, Chan HH, Hermann JK, Jaramillo TC, Machado AG, Baker KB, Dana H. (2021) Cellular-resolution monitoring of ischemic stroke pathologies in the rat cortex. Biomed Opt Express 16;12(8):4901-4919. PMID: 34513232; PMCID: PMC8407830

Jin C, Kong L, Dana H, Xie H, Cao L, Jin G, Dai Q. (2020) Advances in point spread function engineering for functional imaging of neural circuits in vivo. J. Phys. D: Appl. Phys. 53 383001

Dana H, Shoham S. (2020). Two-photo microscopy in the mammalian Brain. In: Handbook of Neurophotonics (Pavone F, Shoham S, eds.) CRC Press, Chapter 3, pp. 55-79.

Das A, Bastian C, Trestan L, Suh J, Dey T, Trapp BD, Baltan S, Dana H. (2020) Reversible Loss of Hippocampal Function in a Mouse Model of Demyelination/Remyelination. Front Cell Neurosci. Jan 22;13:588. PMID: 32038176; PMCID: PMC6987410

Dana H, Sun Y, Mohar B, Hulse BK, Kerlin AM, Hasseman JP, Tsegaye G, Tsang A, Wong A, Patel R, Macklin JJ, Chen Y, Konnerth A, Jayaraman V, Looger LL, Schreiter ER, Svoboda K, Kim DS. (2019) High-performance calcium sensors for imaging activity in neuronal populations and microcompartments. Nat Methods 16(7):649-657. PMID: 31209382

Moeyaert B, Holt G, Madangopal R, Perez-Alvarez A, Fearey BC, Trojanowski NF, Ledderose J, Zolnik TA, Das A, Patel D, Brown TA, Sachdev RNS, Eickholt BJ, Larkum ME, Turrigiano GG, Dana H, Gee CE, Oertner TG, Hope BT, Schreiter ER. (2018) Improved methods for marking active neuron populations. Nat Commun. 25;9(1):4440. PMID: 30361563; PMCID: PMC6202339

Dana H, Novak O, Guardado-Montesino M, Fransen JW, Hu A, Borghuis BG, Guo C, Kim DS, Svoboda K. (2018) Thy1 transgenic mice expressing the red fluorescent calcium indicator jRGECO1a for neuronal population imaging in vivo. PLoS One Oct 11;13(10):e0205444. PMID: 30308007 PMCID: PMC6181368

Shen Y, Dana H, Abdelfattah AS, Patel R, Shea J, Molina RS, Rawal B, Rancic V, Chang YF, Wu L, Chen Y, Qian Y, Wiens MD, Hambleton N, Ballanyi K, Hughes TE, Drobizhev M, Kim DS, Koyama M, Schreiter ER, Campbell RE. (2018) A genetically encoded Ca2+ indicator based on circularly permutated sea anemone red fluorescent protein eqFP578. BMC Biol. 16(1):9. PMID: 29338710; PMCID: PMC5771076

Sun Y, Nern A, Franconville R, Dana H, Schreiter ER, Looger LL, Svoboda K, Kim DS, Hermundstad AM, Jayaraman V. (2017) Neural signatures of dynamic stimulus selection in Drosophila. Nat Neurosci. 20(8):1104-1113. PMID: 28604683

Dana H, Mohar B, Sun Y, Narayan S, Gordus A, Hasseman JP, Tsegaye G, Holt GT, Hu A, Walpita D, Patel R, Macklin JJ, Bargmann CI, Ahrens MB, Schreiter ER, Jayaraman V, Looger LL, Svoboda K, Kim DS. (2016) Sensitive red protein calcium indicators for imaging neural activity. Elife Mar 24;5. pii: e12727. PMID: 27011354; PMCID: PMC4846379

Chu J, Oh Y, Sens A, Ataie N, Dana H, Macklin JJ, Laviv T, Welf ES, Dean KM, Zhang F, Kim BB, Tang CT, Hu M, Baird MA, Davidson MW, Kay MA, Fiolka R, Yasuda R, Kim DS, Ng HL, Lin MZ. (2016) A bright cyan-excitable orange fluorescent protein facilitates dual-emission microscopy and enhances bioluminescence imaging in vivo. Nat Biotechnol. 34(7):760-7. PMID: 27240196; PMCID: PMC4942401

Paluch-Siegler S, Mayblum T, Dana H, Brosh I, Gefen I, Shoham S. (2015) All-optical bidirectional neural interfacing using hybrid multiphoton holographic optogenetic stimulation. Neurophotonics 2(3):031208. PMID: 26217673; PMCID: PMC4512959

Fosque BF*, Sun Y*, Dana H*, Yang CT, Ohyama T, Tadross MR, Patel R, Zlatic M, Kim DS, Ahrens MB, Jayaraman V, Looger LL, Schreiter ER. (2015) Neural circuits. Labeling of active neural circuits in vivo with designed calcium integrators. Science 347(6223):755-60. PMID: 25678659

Dana H, Chen TW, Hu A, Shields BC, Guo C, Looger LL, Kim DS, Svoboda K. (2014) Thy1-GCaMP6 transgenic mice for neuronal population imaging in vivo. PLoS One 9(9):e108697. PMID: 25250714; PMCID: PMC4177405

Thestrup T, Litzlbauer J, Bartholomäus I, Mues M, Russo L, Dana H, Kovalchuk Y, Liang Y, Kalamakis G, Laukat Y, Becker S, Witte G, Geiger A, Allen T, Rome LC, Chen TW, Kim DS, Garaschuk O, Griesinger C, Griesbeck O. (2014) Optimized ratiometric calcium sensors for functional in vivo imaging of neurons and T lymphocytes. Nat Methods 11(2):175-82. PMID: 24390440

Dana H*, Marom A*, Paluch S, Dvorkin R, Brosh I, Shoham S. (2014) Hybrid multiphoton volumetric functional imaging of large-scale bioengineered neuronal networks. Nat Commun. 5:3997. PMID: 24898000; PMCID: PMC4113029

Dana H, Kruger N, Ellman A, Shoham S. (2013) Line temporal focusing characteristics in transparent and scattering media. Opt Express 21(5):5677-87. PMID: 23482141

Dana H, Shoham S. (2012) Remotely scanned multiphoton temporal focusing by axial grism scanning. Opt Lett. 37(14):2913-5. PMID: 22825176

Szameit A*, Shechtman Y*, Osherovich E*, Bullkich E, Sidorenko P, Dana H, Steiner S, Kley EB, Gazit S, Cohen-Hyams T, Shoham S, Zibulevsky M, Yavneh I, Eldar YC, Cohen O, Segev M. (2012) Sparsity-based single-shot subwavelength coherent diffractive imaging. Nat Mater. 11(5):455-9. PMID: 22466747

Dana H, Shoham S. (2011) Numerical evaluation of temporal focusing characteristics in transparent and scattering media. Opt Express 19(6):4937-48. PMID: 21445129

No news currently found.