Assistant Professor, Molecular Medicine, CCLCM-CWRU
Location: Cleveland Clinic Main Campus
The Dana lab records and manipulates neurons in the brain using nonlinear microscopy and protein sensors. Our goals are to better understand the role of brain circuits and the efficacy of novel neuroprotective traetments in rodent models of neurodegenerative and neurological conditions.
Hod Dana received his B.Sc. (summa cum laude) and Ph.D. degrees in Biomedical Engineering from the Technion – Israel Institute of Technology. During his PhD work he developed advance nonlinear microscopy methods for recording of large-scale neuronal activity using a method called temporal focusing. Dr. Dana did his post-doctoral training with the GENIE project at the Howard Hughes Medical Institute Janelia Research Campus. As a postdoctoral researcher, he was involved in the development and testing of new calcium sensors for recording neuronal activity, such as the GCaMP7 sensor, the red sensors jRGECO1 and jRCaMP1, and the calcium integrator CaMPARI. He then joined the Department of Neurosciences at the Lerner Research Institute of the Cleveland Clinic as an Assistant Professor in 2017. He is also an Assistant Professor of Molecular Medicine at the Cleveland Clinic Lerner College of Medicine of Case Western Reserve University.
Dr. Dana is a co-instructor of the Cold Spring Harbor Laboratory summer course on Imaging the Structure and Function of the Nervous system.
"Progress in science depends on new techniques, new discoveries, and new ideas, probably in that order" - Sydney Brenner
In the lab, we develop new methods and platforms for enabling large-scale recording and manipulation of neurons in the living brain. We believe that this approach is essential for a better understanding of how the spatially-distributed and highly-interconnected circuits in the mammalian brain integrate sensory input and transform it into motor action, especially in the context of neurodegeneration or various neurologic conditions. This type of computation is also involved in almost any behavior or decision-making process we do in our everyday life; therefore, we want to gain a deeper understanding of its nature. We work with state-of-the-art optical tools, such as ultrafast lasers, nonlinear microscopy and high-harmonic generation, as well as with novel protein sensors and actuators for detecting and manipulating the brain activity.
The research in the lab is interdisciplinary and collaborative in its nature, and combines approaches from different fields, like engineering, physics, structural biology, and systems neuroscience. Our current projects focus on changes to the brain activity following an injury, and use rodent models of myelin injury, traumatic brain injury, and stroke injury. Another project develop the method and application for recording detailed brain activity from freely-moving rodents.
Das A, Holden S., Borovicka J., Icardi J., O’Niel A., Chaklai A., Patel D., Patel R., Kaech Petrie S., Raber J., and Dana H. (2023), “Recording large-scale, cellular-resolution neuronal activity from freely-moving mice”, Nature Communications, 14, 6399 .
Chornyy S, Borovicka JA, Patel D, Shin MK, Vazquez-Rosa E, Miller E, Wilson B, Pieper AA, and Dana H. (2023) Longitudinal in vivo monitoring of axonal degeneration after brain injury. Cell Reports Methods, (3), 5, 100481(2023).
Li B., Kong L. Piatkevich KD, and Dana H. (2023) Editorial: Large-scale recording of neuronal activity at high spatiotemporal resolutions and applications in neuroscience. Frontiers in Neuroscience 17 (Editorial summary of a special issue on Large-scale recording of neuronal activity at high spatiotemporal resolutions and applications in neuroscience).
Das A*, Margevicius D*, Borovicka J, Icardi J, Patel D, Paquet ME, and Dana H. (2023) Enhanced detection sensitivity of neuronal activity patterns using CaMPARI1 vs. CaMPARI2. Frontiers in Neuroscience 16 -2022. PMID: 36704000, PMCID: PMC9871923
Chornyy S, Das A, Borovicka JA, Patel D, Chan HH, Hermann JK, Jaramillo TC, Machado AG, Baker KB, Dana H. (2021) Cellular-resolution monitoring of ischemic stroke pathologies in the rat cortex. Biomed Optics Express 16;12(8):4901-4919. PMID: 34513232; PMCID: PMC8407830
Jin C, Kong L, Dana H, Xie H, Cao L, Jin G, Dai Q. (2020) Advances in point spread function engineering for functional imaging of neural circuits in vivo. Journal of Physics D: Applied Physics 53 38300
Dana H, Shoham S. (2020). Two-photon microscopy in the mammalian Brain. In: Handbook of Neurophotonics (Pavone F, Shoham S, eds.) CRC Press, Chapter 3, pp. 55-79.
Das A, Bastian C, Trestan L, Suh J, Dey T, Trapp BD, Baltan S, Dana H. (2020) Reversible Loss of Hippocampal Function in a Mouse Model of Demyelination/Remyelination. Frontiers in Cellular Neuroscience Jan 22;13:588. PMID: 32038176; PMCID: PMC6987410
Dana H, Sun Y, Mohar B, Hulse BK, Kerlin AM, Hasseman JP, Tsegaye G, Tsang A, Wong A, Patel R, Macklin JJ, Chen Y, Konnerth A, Jayaraman V, Looger LL, Schreiter ER, Svoboda K, Kim DS. (2019) High-performance calcium sensors for imaging activity in neuronal populations and microcompartments. Nature Methods 16(7):649-657. PMID: 31209382
Moeyaert B, Holt G, Madangopal R, Perez-Alvarez A, Fearey BC, Trojanowski NF, Ledderose J, Zolnik TA, Das A, Patel D, Brown TA, Sachdev RNS, Eickholt BJ, Larkum ME, Turrigiano GG, Dana H, Gee CE, Oertner TG, Hope BT, Schreiter ER. (2018) Improved methods for marking active neuron populations. Nature Communications 25;9(1):4440. PMID: 30361563; PMCID: PMC6202339
Dana H, Novak O, Guardado-Montesino M, Fransen JW, Hu A, Borghuis BG, Guo C, Kim DS, Svoboda K. (2018) Thy1 transgenic mice expressing the red fluorescent calcium indicator jRGECO1a for neuronal population imaging in vivo. PLoS One Oct 11;13(10):e0205444. PMID: 30308007 PMCID: PMC6181368
Shen Y, Dana H, Abdelfattah AS, Patel R, Shea J, Molina RS, Rawal B, Rancic V, Chang YF, Wu L, Chen Y, Qian Y, Wiens MD, Hambleton N, Ballanyi K, Hughes TE, Drobizhev M, Kim DS, Koyama M, Schreiter ER, Campbell RE. (2018) A genetically encoded Ca2+ indicator based on circularly permutated sea anemone red fluorescent protein eqFP578. BMC Biology 16(1):9. PMID: 29338710; PMCID: PMC5771076
Sun Y, Nern A, Franconville R, Dana H, Schreiter ER, Looger LL, Svoboda K, Kim DS, Hermundstad AM, Jayaraman V. (2017) Neural signatures of dynamic stimulus selection in Drosophila. Nature Neuroscience 20(8):1104-1113. PMID: 28604683
Dana H, Mohar B, Sun Y, Narayan S, Gordus A, Hasseman JP, Tsegaye G, Holt GT, Hu A, Walpita D, Patel R, Macklin JJ, Bargmann CI, Ahrens MB, Schreiter ER, Jayaraman V, Looger LL, Svoboda K, Kim DS. (2016) Sensitive red protein calcium indicators for imaging neural activity. Elife Mar 24;5. pii: e12727. PMID: 27011354; PMCID: PMC4846379
Chu J, Oh Y, Sens A, Ataie N, Dana H, Macklin JJ, Laviv T, Welf ES, Dean KM, Zhang F, Kim BB, Tang CT, Hu M, Baird MA, Davidson MW, Kay MA, Fiolka R, Yasuda R, Kim DS, Ng HL, Lin MZ. (2016) A bright cyan-excitable orange fluorescent protein facilitates dual-emission microscopy and enhances bioluminescence imaging in vivo. Nature Biotechnology 34(7):760-7. PMID: 27240196; PMCID: PMC4942401
Paluch-Siegler S, Mayblum T, Dana H, Brosh I, Gefen I, Shoham S. (2015) All-optical bidirectional neural interfacing using hybrid multiphoton holographic optogenetic stimulation. Neurophotonics 2(3):031208. PMID: 26217673; PMCID: PMC4512959
Fosque BF*, Sun Y*, Dana H*, Yang CT, Ohyama T, Tadross MR, Patel R, Zlatic M, Kim DS, Ahrens MB, Jayaraman V, Looger LL, Schreiter ER. (2015) Labeling of active neural circuits in vivo with designed calcium integrators. Science 347(6223):755-60. PMID: 25678659
Dana H, Chen TW, Hu A, Shields BC, Guo C, Looger LL, Kim DS, Svoboda K. (2014) Thy1-GCaMP6 transgenic mice for neuronal population imaging in vivo. PLoS One 9(9):e108697. PMID: 25250714; PMCID: PMC4177405
Thestrup T, Litzlbauer J, Bartholomäus I, Mues M, Russo L, Dana H, Kovalchuk Y, Liang Y, Kalamakis G, Laukat Y, Becker S, Witte G, Geiger A, Allen T, Rome LC, Chen TW, Kim DS, Garaschuk O, Griesinger C, Griesbeck O. (2014) Optimized ratiometric calcium sensors for functional in vivo imaging of neurons and T lymphocytes. Nature Methods 11(2):175-82. PMID: 24390440
Dana H*, Marom A*, Paluch S, Dvorkin R, Brosh I, Shoham S. (2014) Hybrid multiphoton volumetric functional imaging of large-scale bioengineered neuronal networks. Nature Communications 5:3997. PMID: 24898000; PMCID: PMC4113029
Dana H, Kruger N, Ellman A, Shoham S. (2013) Line temporal focusing characteristics in transparent and scattering media. Optics Express 21(5):5677-87. PMID: 23482141
Dana H, Shoham S. (2012) Remotely scanned multiphoton temporal focusing by axial grism scanning. Optics Letters 37(14):2913-5. PMID: 22825176
Szameit A*, Shechtman Y*, Osherovich E*, Bullkich E, Sidorenko P, Dana H, Steiner S, Kley EB, Gazit S, Cohen-Hyams T, Shoham S, Zibulevsky M, Yavneh I, Eldar YC, Cohen O, Segev M. (2012) Sparsity-based single-shot subwavelength coherent diffractive imaging. Nature Materials 11(5):455-9. PMID: 22466747
Dana H, Shoham S. (2011) Numerical evaluation of temporal focusing characteristics in transparent and scattering media. Optics Express 19(6):4937-48. PMID: 21445129
Our education and training programs offer hands-on experience at one of the nationʼs top hospitals. Travel, publish in high impact journals and collaborate with investigators to solve real-world biomedical research questions.Learn More
The new method will be used in preclinical research with the goal of improving care for patients with neurological disease.