Research Core Services
The Lerner Research Institute's Core Services seek to facilitate and advance research throughout Cleveland Clinic by providing technologies that support basic, translational, and clinical research.
Cell Culture Core
Frequently Asked Questions
Q. What is the cryopreservation capacity in the cell Culture lab?
A. Currently we can store 10,000 vials.
Q. How should I prepare my samples for mycoplasma testing?
A. This information can be found on the mycoplasma order form.
Q. Should I trypsinize the cells for mycoplasma testing?
A. No. Scrape cells and supply 5mL in a tube.
Q. I'm new to cell culture, where do I find information on tissue culture techniques?
A. The Cell Core has several protocols on cell culture:
- Aseptic techniques.
- Recovery of cells from liquid nitrogen.
- Culturing cells- monolayer and suspension.
- Performing a viable count.
- Storage of cells in liquid nitrogen.
- Quality Control.
- Specialized techniques.
Q. Is there a facility for large-scale cell culture?
A. The Cell Culture Core has the capacity to grow large volumes of cells, both monolayer, suspension, and insect cells.
Q. In addition to the cell services listed, will the core provide "special cell cultures" that are not on the list?
A. Yes, The core will accommodate any special orders. The researcher would need to discuss this in advance with Cell Services Personnel.
Q. How does the cost for cell services compare with other institutes and outside companies?
A. Our charges are competitively priced. We charge less than other institutes for certain services and we are much cheaper than outside companies offering the same service.
Q. Why should I purchase cells from the Cell Core if I can get them free from my colleagues?
A. Cells obtained from outside sources may not always be as healthy as you think. All cells from the Cell Services Core are stored at early passages and are regularly tested for mycoplasma and endotoxins.