Research Core Services
The Lerner Research Institute's Core Services seek to facilitate and advance research throughout Cleveland Clinic by providing technologies that support basic, translational, and clinical research.
Molecular Screening Core
Molecular Screening Core (MSC) provides the researchers at CCF and other biomedical research centers in Cleveland area with the expertise, technology, instrumentation and reagents necessary for the screening of the library of small molecules for biologically active compounds in a variety of readout systems. MSC owns the diverse historical chemical library of 34,000 compounds manufactured by Chembridge Corporation, world recognized leader in this field. The core can provide help with the adjustment of readout systems for both cell-based and biochemical assays and establishment of screening conditions based on a pilot small-scale screening. If successful, the established system is used for a full-scale library screening in 96-well format followed by generation of the database with the results. MSC is equipped with laminar flow hood, CO2 incubators and Multidrop 384 (Thermo/Labsystems) to assist in plating and growing cells in 96-well format. Wallac 1420 VICTOR2 multilabel reader can be used to collect the data in variety of readout systems. Additionally MSC can provide the information about cytotoxicity of selected hits.
MSC also offers help with data processing and planning further steps in optimization of isolated chemicals, including primary chemoinformatics analysis, generation of virtual libraries of analogues and consulting on the tactics of compound characterization, including connection with outside partners.