Dr. Jung and his team defined the molecular mechanisms that underlie fetal brain calcification caused by Zika virus infection, most notably identifying the role of the protease NS3 in driving the virus-induced abnormal brain development.
Researchers have described for the first time how Zika virus (ZIKV) causes one of the most common birth defects associated with prenatal infection, called brain calcification, according to new study findings published in Nature Microbiology.
The findings may reveal novel strategies to prevent prenatal ZIKV brain calcification and offer important insights into how calcifications form in other congenital infections.
“Brain calcification has been linked to several developmental defects in infants, including motor disorders, cognitive disability, eye abnormalities, hearing deficits and seizures, so it’s important to better understand the mechanisms of how they develop,” said Jae Jung, PhD, director of Cleveland Clinic’s Global Center for Pathogen Research & Human Health and lead author on the study. Dr. Jung, who is also chair of the Department of Cancer Biology, joined Cleveland Clinic from the University of Southern California in July 2020.
Utilizing ZIKV-positive fetal brain specimens and preclinical disease models, the research team identified the protein BMP2 (bone morphogenetic protein 2) and the protease NS3 (nonstructural protein 3) as central players in ZIKV brain calcification.
Scans of ZIKV-positive brains collected and donated from Brazil during the 2015-2016 Zika outbreak revealed that cells called pericytes, which wrap around blood capillaries in the brain, collect around calcified lesions and have high concentrations of viral genetic material, suggesting a key role in infection-induced calcification.
“Several cell types have been interrogated for their involvement in ZIKV infection and pathology, but to our knowledge, ours is the first study to investigate the role of pericytes,” said Weiqiang Chen, PhD, a research associate in Dr. Jung’s lab and the first author on the study.
The researchers infected fetal-derived pericytes with ZIKV and found that the BMP2 gene, which encodes the BMP2 protein, and several downstream osteogenic genes (related to bone formation) were expressed at higher levels and resulted in calcification. The team went on to conduct protein expression analyses to understand precisely how these changes induce calcification.
“We discovered that during ZIKV infection, some of the BMP family of genes and associated proteins function differently than during normal embryogenesis and development,” commented Dr. Chen.
Normally, BMPs activate specific proteins (the SMAD protein family) in order to modulate expression of osteogenic genes. Dr. Jung and his team showed here, however, that during ZIKV infection, a viral protease (an enzyme) called NS3 transforms BMP2 into a mature and biologically active form that enhances osteogenic gene expression. Effectively, NS3 activates BMP2 signaling pathways to trigger calcification and faulty brain development.
More research will be critical, but as Dr. Chen explained, the study findings suggest that perhaps targeting NS3, either by preventing its expression or its binding with BMP2, may help to prevent ZIKV brain calcification in utero.
Dr. Chen is supported by a Pathway to Independence Award (K99/R00) from the National Institute of Dental and Craniofacial Research, part of the National Institutes of Health.
Director, Global Center for Pathogen & Human Health Research
Department Chair, Cancer Biology
Director, Infection Biology
Betsy B. deWindt Endowed Chair in Cancer Biology
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